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Notch2-dependent classical dendritic cells orchestrate intestinal immunity to attaching-and-effacing bacterial pathogens

Nature Immunology volume 14, pages 937–948 (2013)Cite this article

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Abstract

Defense against attaching-and-effacing bacteria requires the sequential generation of interleukin 23 (IL-23) and IL-22 to induce protective mucosal responses. Although CD4+ and NKp46+ innate lymphoid cells (ILCs) are the critical source of IL-22 during infection, the precise source of IL-23 is unclear. We used genetic techniques to deplete mice of specific subsets of classical dendritic cells (cDCs) and analyzed immunity to the attaching-and-effacing pathogen Citrobacter rodentium. We found that the signaling receptor Notch2 controlled the terminal stage of cDC differentiation. Notch2-dependent intestinal CD11b+ cDCs were an obligate source of IL-23 required for survival after infection with C. rodentium, but CD103+ cDCs dependent on the transcription factor Batf3 were not. Our results demonstrate a nonredundant function for CD11b+ cDCs in the response to pathogens in vivo.

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Figure 1: Zbtb46-GFP identifies intestinal cDC populations.
Figure 2: Zbtb46+ cDCs are essential for survival after infection with C. rodentium.
Figure 3: Canonical Notch2 signaling is required for the development of splenic and intestinal CD11b+ cDCs.
Figure 4: Notch2 controls the terminal differentiation of CD11b+ and DEC-205+ cDCs.
Figure 5: LTβR signaling mediates the homeostatic population expansion of Notch2-dependent cDCs.
Figure 6: Notch2-dependent CD11b+ cDCs are essential for host defense against infection with C. rodentium.
Figure 7: Notch2-dependent cDCs are dispensable for colonic wound repair.
Figure 8: Notch2-dependent CD11b+ cDCs regulate IL-23-dependent antimicrobial responses to C. rodentium.

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Acknowledgements

We thank B. Sleckman (Washington University in St. Louis) for Nik−/− mice; T. Watts (University of Toronto) for Ifnar1−/− mice; J. Boothroyd (Stanford University) for the plasmid PRU-FLuc-GFP; the Immunological Genome Project consortium for use of their database54; and the Alvin J. Siteman Cancer Center at Washington University School of Medicine for use of the Center for Biomedical Informatics and Multiplex Gene Analysis Genechip Core Facility. Supported by the Howard Hughes Medical Institute, the US National Institutes of Health (AI076427-02 to K.M.M., R01 GM55479 to R.K., R01 DE021255-01 and U01 AI095542-01 to M.C., R01 DK071619 to T.S.S. and R01 DK064798 to R.D.N.), the US Department of Defense (W81XWH-09-1-0185 to K.M.M.), the American Heart Association (12PRE8610005 to A.T.S. and 12PRE12050419 to W.K.), the Canadian Institutes of Health Research (MOP 67157 to J.L.G. and FRN 11530 to C.J.G.) and the National Cancer Institute (P30 CA91842 for the Alvin J. Siteman Cancer Center).

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Authors and Affiliations

  1. Department of Pathology and Immunology, Washington University in St. Louis, School of Medicine, St. Louis, Missouri, USA

    Ansuman T Satpathy, Carlos G Briseño, Jacob S Lee, Nicholas A Manieri, Wumesh KC, Xiaodi Wu, Stephanie R Thomas, Wan-Ling Lee, Christina Song, Theresa L Murphy, Thaddeus S Stappenbeck, Marco Colonna & Kenneth M Murphy

  2. Department of Immunology, University of Toronto, Toronto, Ontario, Canada

    Dennis Ng, Cynthia J Guidos & Jennifer L Gommerman

  3. Department of Developmental Biology, Washington University in St. Louis, School of Medicine, St. Louis, Missouri, USA.,

    Mustafa Turkoz & Raphael Kopan

  4. Department of Internal Medicine, Washington University in St. Louis, School of Medicine, St. Louis, Missouri, USA

    Keely G McDonald & Rodney D Newberry

  5. Laboratory of Molecular Immunology, The Rockefeller University, New York, New York, USA

    Matthew M Meredith & Michel C Nussenzweig

  6. Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, Ontario, Canada

    Cynthia J Guidos

  7. Department of Immunology, Genentech, South San Francisco, California, USA

    Wenjun Ouyang

  8. Howard Hughes Medical Institute, The Rockefeller University, New York, New York, USA

    Michel C Nussenzweig

  9. Howard Hughes Medical Institute, Washington University in St. Louis, School of Medicine, St. Louis, Missouri, USA.,

    Kenneth M Murphy

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  1. Ansuman T Satpathy
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Contributions

A.T.S. and K.M.M. designed the study; A.T.S., C.G.B., J.S.L. and C.S. did experiments related to infection with C. rodentium, with guidance from W.O., M.C. and K.M.M.; A.T.S., C.G.B. and D.N. did experiments related to Ltbr−/− mice, with guidance from C.J.G. and J.L.G.; N.A.M. did experiments related to wound healing, with guidance from T.S.S.; A.T.S. and X.W. did microarray analysis; A.T.S., S.R.T., W.K., W.-L.L., M.T., T.L.M. and K.G.M. did experiments related to cDC development in mice deficient in Notch2, Irf4 or Batf3, with guidance from R.K., R.D.N. and K.M.M.; A.T.S., C.G.B. and M.M.M. did experiments with Zbtb46gfp and Zbtb46DTR mice, with guidance from M.C.N. and K.M.M.; and A.T.S. and K.M.M. wrote the manuscript with contributions from all authors.

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Correspondence to Kenneth M Murphy.

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W.O. is an employee of Genentech.

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Satpathy, A., Briseño, C., Lee, J. et al. Notch2-dependent classical dendritic cells orchestrate intestinal immunity to attaching-and-effacing bacterial pathogens. Nat Immunol 14, 937–948 (2013). https://doi.org/10.1038/ni.2679

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