Abstract
We have produced extracts of frog eggs that can perform multiple cell cycles in vitro. Destruction of the endogenous messenger RNA arrests the extracts in interphase. The addition of exogenous cyclin mRNA is sufficient to produce multiple cell cycles. The newly synthesized cyclin protein accumulates during each interphase and is degraded at the end of each mitosis.
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References
Masui, Y. & Markert, C. J. exp. Zool. 177, 129–146 (1971).
Reynhout, J. K. & Smith, L. D. Devl Biol. 38, 394–400 (1974).
Gerhart, J., Wu, M. & Kirschner, M. W. J. Cell Biol. 98, 1247–1255 (1984).
Kishimoto, T. & Kanatani, H. Gen. comp. Endocr. 33, 41–44 (1977).
Gerhart, J., Wu, M., Cyert, M. & Kirschner, M. Cytobios 43, 335–347 (1985).
Dunphy, W. G., Brizuela, L., Beach, D. & Newport J. Cell 54, 423–431 (1988).
Gautier, J., Norbury, C., Lohka, M., Nurse, P. & Maller, J. Cell 54, 433–439 (1988).
Lee, M. & Nurse, P. Trends Genet. 4, 287–290 (1988).
Labbe, J. C., Lee, M. G., Nurse, P., Picard, A. & Doree, M. Nature 355, 251–254 (1988).
Draetta, G. & Beach, D. Cell 54, 17–26 (1988).
Arion, D., Meijer, L., Brizuela, L. & Beach, D. Cell 55, 371–378 (1988).
Masui, Y. J. exp. Zool. 187, 141–147 (1974).
Watanabe, N. & Shimada, H. Cell Differ. 13, 239–245 (1983).
Miake-Lye, R., Newport, J. & Kirschner, M. W. J. Cell Blol. 97, 81–91 (1983).
Evans, T., Rosenthal, E. T., Youngblom, J., Distel, D. & Hunt, T. Cell 33, 389–396 (1983).
Swenson, K. I., Farrell, K. M. & Ruderman, J. V. Cell 47, 861–870 (1986).
Pines, J. & Hunt, T. EMBO J. 6, 2987–2995 (1987).
Standart, N., Minshull, J., Pines, J. & Hunt, T. Devl Biol. 124, 248–258 (1987).
Minshull, J., Blow, J. & Hunt, T. Cell 56, 947–956 (1989).
Lehner, C. F. & O'Farrell, P. Cell 56, 957–968 (1989).
Booher, R. & Beach, D. EMBO J. 7, 2321–2327 (1988).
Solomon, M., Booher, R., Kirschner, M. & Beach, D. Cell 54, 738–739 (1988).
Goebl, M. & Byers, B. Cell 54, 739–740 (1988).
Hagan, I., Hayles, J. & Nurse, P. J. Cell Sci. 91, 587–595 (1988).
Lohka, M. & Masui, Y. J. Cell Biol. 98, 1222–1230 (1984).
Blow, J. & Watson, J. EMBO J. 6, 1997–2002 (1987).
Cyert, M. S. & Kirschner, M. W. Cell 53, 185–195 (1988).
Hutchison, C. J., Cox, R., Drepaul, R. S., Gomperts, M. & Ford, C. C. EMBO J. 6, 2003–2010 (1987).
Miake-Lye, R. & Kirschner, M. W. Cell 41, 165–175 (1985).
Lohka, M. J. & Maller, J. L. J. Cell Biol. 101, 518–523 (1985).
Newport, J. & Spann, T. Cell 48, 219–230 (1987).
Blackburn, P., Wilson, G. & Moore, S. J. biol. Chem. 252, 5904–5910 (1977).
Murray, A. W., Solomon, M. J. & Kirschner, M. K. Nature 339, 280–286 (1989).
Wagenaar, E. B. Expl Cell Res. 144, 393–403 (1983).
Karsenti, E., Bravo, R. & Kirschner, M. W. Devl Biol. 119, 442–453 (1987).
Edgar, B. A. & O'Farrell, P. H. Cell 57, 177–187 (1989).
Russell, P. & Nurse, P. Cell 45, 145–153 (1986).
Hara, K., Tydeman, P. & Kirschner, M. W. Proc. natn. Acad Sci. U.S.A. 77, 462–466 (1980).
Newport, J. W. & Kirschner, M, W. Cell 37, 731–742 (1984).
Hartwell, L. H. J. Cell Biol. 77, 627–637 (1978).
Croy, R. G. & Pardee, A. B. Proc. natn. Acad. Sci. U.S.A. 80, 4699–4703 (1983).
Campisi, J., Morreo, G. & Pardee, A. B. Expl Cell Res. 152, 459–466 (1984).
Nurse, P. & Bissett, Y. Nature 292, 558–560 (1981).
Cross, F. Molec. Cell Biol. 8, 4675–4684 (1988).
Nash, R., Tokiwa, G., Anand, S., Erickson, K. & Futcher, A. B. EMBO J. 7, 4335–4346 (1988).
Newport, J. & Kirschner, M. W. Cell 30, 687–696 (1982).
Gurdon, J. B. J. Embryol. exp. Morph. 36, 523–540 (1976).
Palter, K. B. & Alberts, B. M. J. biol. Chem. 254, 11160–11169 (1979).
Anderson, C. W., Baum, P. R. & Gesteland, R. F. J. Virol. 12, 241–252 (1973).
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Murray, A., Kirschner, M. Cyclin synthesis drives the early embryonic cell cycle. Nature 339, 275–280 (1989). https://doi.org/10.1038/339275a0
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DOI: https://doi.org/10.1038/339275a0
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