Abstract
Myeloid precursors can be grown in vitro in the presence of specific growth factors; however, their expansion is limited by a competing process of terminal differentiation1,2. Proto-oncogenes seem to be involved in cellular proliferation and/or differentiation and may also play a role in the myelopoietic process3,4. Murine myeloid precursors which are grown in vitro with growth factors respond with augmented self-renewal upon infection with recombinant retroviruses carrying the v-myc or v-src oncogenes5–11, suggesting a synergism or complementation between some viral oncogenes (v-onc) and certain growth factors. We now show that the combination of two v-onc genes (raf and myc) induces the selective proliferation of monocytic cells from fresh murine bone marrow (BM) in the absence of a specific growth factor supplement. Depending on the culture conditions these cells can either differentiate and cease to proliferate or grow continuously, thus mimicking the alternative pathways that can be followed by committed BM stem cells in vivo.
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References
Neumann, C. & Sorg, C. Eur. J. Immun. 10, 834–840 (1980).
Tushinski, R. J. et al. Cell 28, 71–81 (1982).
Bishop, J. M. A. Rev.Biochem. 52, 301–354 (1983).
Müller, R. & Verma, I. M. Curr. Topics Microbiol. Immun. 112, 73–115 (1984).
Spooncer, E., Boettinger, D. & Dexter, T. M. Nature 310, 228–230 (1984).
Adkins, B., Leutz, A. & Graf, T. Cell 39, 439–445 (1984).
Boettinger, D., Anderson, S. & Dexter, T. M. Cell 30, 763–772 (1984).
Vennstroem, B. et al. EMBO J. 3, 3223–3229 (1984).
Gazzolo, G., Moscovici, C. & Moscovici, M. G. Cell 16, 627–638 (1979).
Graf, T. & Beug, H. Biochim. biophys. Acta 516, 269–299 (1978).
Beug, H., Heyman, M. J. & Graf, T. EMBO J. 1, 1069–1073 (1982).
Rapp, U. R. & Nowinski, R. C. J. Virol. 18, 411–417 (1976).
Bunte, T. et al. EMBO J. 3, 1919–1924 (1984).
Schultz, A. M., Copeland, T. D., Mark, G. E., Rapp, U. R. & Oroszlan, S. Virology 146, 78–89 (1985).
Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).
Durban, E. & Boettinger, D. Proc. natn. Acad. Sci. U.S.A. 78, 3600–3604 (1981).
Ihle, J. N., Keller, J., Rein, A., Cleveland, J. & Rapp, U. in Cancer Cells Growth Factors and Transformation Vol. 3 (eds Feramisco, J., Ozanne, B. & Stiles, C.) 211–219 (Cold Spring Harbor Laboratory, New York, 1985).
Rapp, U. R., Cleveland, J. L., Brightman, K., Scott, A. & Ihle, J. N. Nature 317, 434–438 (1985).
Vogel, S. & Rosenstreich, L. Lymphokines 3, 169–182 (1981).
Springer, T., Galfre, G., Secher, D. S. & Milstein, C. Eur. J. Immun. 9, 301–309 (1979).
Unkeless, J. C., Mellman, L. S., McGettigan, M. & Plutner, H. in Heterogeneity ofMononuclear Phagocytes (eds Forster, O. & Landy, M.) 91–96 (Academic, London, 1981).
Springer, T., Galfre, G., Scher, D. S. & Milstein, C. Eur. J. Immun. 8, 539–551 (1978).
Habu, S. et al. J. Immun. 125, 2284–2288 (1980).
Austyn, J. M. & Gordon, S. Eur. J. Immun. 10, 805–812 (1981).
Mathieson, B. J., Sharrow, S. O., Rosenberg, Y. & Hammerling, U. Nature 289, 179–181 (1981).
Potter, T. A., Hogarth, P. M. & McKenzie, I. F. C. Eur. J. Immun. 10, 899–903 (1980).
Pierres, A. et al. J. Immun. 132, 2775–2782 (1984).
Versteegen, R. J. & Oroszlan, S. J. Virol. 33, 983–992 (1980).
Schlick, E., Hartung, K. & Chirigos, M. A. Int. J. Immunopharmac. 6, 407–418 (1984).
Rapp, U. R., Reynolds, R. H. Jr & Stephenson, J. R. J. Virol. 45, 914–924 (1983).
Rapp, U. R. et al. Recent Results Cancer Res. 99, 221–235 (1985).
Rapp, U. R. et al. J. Virol. 55, 23–33 (1985).
Stringer, J. R., Holland, L. E., Swanstrom, R. I., Rivo, K. & Wagner, E. K. J. Virol. 21, 889–901 (1977).
McMaster, C. K. & Carmichael, G. G. Proc. natn. Acad. Sci. U.S.A. 74, 4835–4838 (1977).
Lautenberger, J. A., Schultz, R. A., Garon, C. T., Tsichlis, P. N. & Papas, T. S. Proc. natn. Acad. Sci. U.S.A. 78, 1518–1522 (1981).
Vennstrom, B., Moscovici, C., Goodman, H. M. & Bishop, J. M. J. Virol. 39, 625–631 (1981).
Bonner, T. I. et al. Nucleic Acids Res. (submitted).
Poiesz, B. J., Ruscetti, F. W., Mier, J. W., Woods, A. M. & Gallo, R. C. Proc. natn. Acad. Sei. U.S.A. 77, 6815–6819 (1980).
Rapp, U. R., Bonner, T. I. & Cleveland, J. L. in Retroviruses in Human Pathology (eds Gallo, R. C., Stehelin, D. & Varnier, O. E.) (Humane, New Jersey, in the press).
Sheiness, D. K., Hughes, S. H., Varmus, H. E., Stubblefield, E. & Bishop, J. M. Virology 105, 415–424 (1980).
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Blasi, E., Mathieson, B., Varesio, L. et al. Selective immortalization of murine macrophages from fresh bone marrow by a raf/myc recombinant murine retrovirus. Nature 318, 667–670 (1985). https://doi.org/10.1038/318667a0
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DOI: https://doi.org/10.1038/318667a0
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