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Isolation of RNA and DNA fragments using diatomaceous earth

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Biotechnology Techniques

Abstract

A series of simple and phenol-free silica-based protocols for isolating and cleaning RNA or DNA fragments from different sources were developed. Cytoplasmic RNA isolated from hybridoma cells by this method was used in RT-PCR. DNA fragments obtained using this protocol were suitable for further subcloning, gene transformation and DNA sequencing. © Rapid Science Ltd. 1998

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Koo, K., Foegeding, P.M. & Swaisgood, H.E. Isolation of RNA and DNA fragments using diatomaceous earth. Biotechnology Techniques 12, 549–552 (1998). https://doi.org/10.1023/A:1008859632378

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  • DOI: https://doi.org/10.1023/A:1008859632378

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