NASBA: A Novel, Isothermal Detection Technology for Qualitative and Quantitative HIV-1 RNA Measurements
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Introduction on Laboratory Tests for Diagnosis of Infectious Diseases and Immunological Disorders
2022, Encyclopedia of Infection and ImmunityNewcastle disease: Evolution of genotypes and the related diagnostic challenges
2010, Infection, Genetics and EvolutionNucleic acid sequence-based amplification methods to detect avian influenza virus
2004, Biochemical and Biophysical Research CommunicationsCitation Excerpt :Without the need for thermal denaturation for DNA strand separation, as required by conventional PCR, the NASBA assay has the advantage of being able to amplify specific single-stranded RNA target sequences in the presence of genomic DNA contaminants [30]. NASBA/ECL is especially suitable for the detection of RNA viruses, such as influenza, foot-and-mouth disease virus, dengue fever virus, human immunodeficiency virus, and cytomegalovirus, among many others [27,29,36–39]. As the end-product of the NASBA reaction is RNA, which tends to be unstable under normal environmental conditions, the possibility of carryover contamination of equipment from previous experiments is minimized.
Comparison of nucleic acid-based detection of avian influenza H5N1 with virus isolation
2003, Biochemical and Biophysical Research CommunicationsCitation Excerpt :We have reported a similar method for calculating cut-off values [9,10,15]. The use of appropriate internal controls for quantifying HIV viral load using the NASBA technique has been described [14] and the technique is applicable to other targets. The dynamic range of the ECL detector was determined using a serial dilution of a known positive sample of avian influenza H5N1.
Rapid and sensitive detection of avian influenza virus subtype H7 using NASBA
2003, Biochemical and Biophysical Research CommunicationsCitation Excerpt :We have reported similar methods for calculating the cut-off value previously [13,14]. The use of appropriate internal controls for quantifying HIV viral load using the NASBA/ECL technique has been described [12] and the technique is applicable to other targets. Analysing the same sample multiple times assessed reproducibility.
A method to detect major serotypes of foot-and-mouth disease virus
2002, Biochemical and Biophysical Research CommunicationsCitation Excerpt :Other users of NASBA systems have reported similar methods for calculating the cut-off value, including 0.01–0.025×IRS [25,36,37] and 200 ECL units [38]. The use of appropriate internal controls for quantifying HIV viral load using the NASBA technique has been described [14] and the technique is applicable to other targets. Analysing the same sample multiple times assessed reproducibility.