Molecular Cell
Volume 38, Issue 6, 25 June 2010, Pages 815-826
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Article
The Activation Potential of MOF Is Constrained for Dosage Compensation

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Summary

The H4K16 acetyltransferase MOF plays a crucial role in dosage compensation in Drosophila but has additional, global functions. We compared the molecular context and effect of MOF in male and female flies, combining chromosome-wide mapping and transcriptome studies with analyses of defined reporter loci in transgenic flies. MOF distributes dynamically between two complexes, the dosage compensation complex and a complex containing MBD-R2, a global facilitator of transcription. These different targeting principles define the distribution of MOF between the X chromosome and autosomes and at transcription units with 5′ or 3′ enrichment. The male X chromosome differs from all other chromosomes in that H4K16 acetylation levels do not correlate with transcription output. The reconstitution of this phenomenon at a model locus revealed that the activation potential of MOF is constrained in male cells in the context of the DCC to arrive at the 2-fold activation of transcription characteristic of dosage compensation.

Highlights

► Dosage compensation results from strong stimulation and counteracting modulation ► The acetyltransferase MOF distributes dynamically between two distinct complexes ► The H4K16 acetylation levels correlate with transcription except for on the male X ► The MOF interactor MBD-R2 is globally involved in transcription stimulation

PROTEINS
DNA
DEVBIO

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