Expression of the zebrafish Staufen gene in the embryo and adult
Section snippets
Sequence analysis
A comparison of the zebrafish Staufen to other known sequences suggests it is an evolutionary intermediate between invertebrate and vertebrate sequences. The amino acid sequence of the Drosophila, human, and zebrafish Staufen are depicted in Fig. 1A and a representation of the double-stranded RNA binding domains (dsRBDs) and tubulin binding domains (TBD) in Fig. 1B The zebrafish staufen 5′ end includes the first dsRBD, which has a 64% identity to Drosophila and is missing in human staufen;
In situ hybridization (whole mount and sectioned tissue)
Whole mount in situ hybridization was performed as previously described (Thisse et al., 1994). In situ hybridization was performed on zebrafish sectioned tissue as previously described (Braat et al., 1999). The anti-sense staufen probe was a 1600 pb fragment produced by linearizing the TOPO TA vector (Invitrogen) with Bam HI and using T7 RNA polymerase for in vitro transcription. The sense probe was linearized with Hind III and transcribed using T3 polymerase. Vasa in situ hybridization on early
References (14)
- et al.
Staufen protein associates with the 3′UTR of bicoid mRNA to form particles that move in a microtubule-dependent manner
Cell
(1994) - et al.
Identification of Miranda protein domains regulating asymmetric cortical localization, cargo binding, and cortical release
Mol. Cell Neurosci.
(1998) - et al.
Staufen: a common component of mRNA transport in oocytes and neurons?
Trends Cell Biol.
(2000) - et al.
Staufen, a gene required to localize maternal RNAs in the Drosophila egg
Cell
(1991) - et al.
Goosecoid expression in neuroectoderm and mesendoderm is disrupted in zebrafish cyclops gastrulas
Dev. Biol.
(1994) - et al.
Cloning and expression of the Zebrafish germ cell nuclear factor
Mol. Reprod. Dev.
(1999) - et al.
A function for kinesin I in the posterior transport of oskar mRNA and Staufen protein
Science
(2000)
Cited by (14)
An Interaction Network of RNA-Binding Proteins Involved in Drosophila Oogenesis
2020, Molecular and Cellular ProteomicsCytoplasmic remodelling and the acquisition of developmental competence in pig oocytes
2007, Animal Reproduction ScienceCitation Excerpt :Among the many RNA-binding proteins identified so far, Staufen has been shown to be involved in RNA localization in Drosophila oocytes (Gavis and Lehmann, 1992; Hachet and Ephrussi, 2004) and Xenopus laevis eggs (Yoon and Mowry, 2004). Expression of Staufen has also been recently demonstrated in the zebrafish embryo (Bateman et al., 2004). The pig orthologue for Staufen has been recently identified (Staufen; EMBL accession number: AJ969068) in our laboratory (Brevini et al., 2005b).
Zebrafish Staufen1 and Staufen2 are required for the survival and migration of primordial germ cells
2006, Developmental BiologyEmerging Roles of RNA‐Binding Proteins in Neurodevelopment
2022, Journal of Developmental BiologyThe crystal structure of Staufen1 in complex with a physiological RNA sheds light on substrate selectivity
2018, Life Science Alliance