Molecular & Cellular Proteomics
Volume 20, 2021, 100119
Technological Innovation and ResourcesIdentification of Endogenous Kinase Substrates by Proximity Labeling Combined with Kinase Perturbation and Phosphorylation Motifs
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open access
Graphical Abstract
Keywords
kinase substrate
BioID (proximity-dependent biotin identification)
phosphoproteomics
Abbreviations
ACN
acetonitrile
AP-MS
affinity-purification mass spectrometry
BioID
proximity-dependent biotin identification
BirA
biotin ligase
CK2
casein kinase 2
GO
gene ontology
HAMMOC
hydroxy-acid-modified metal oxide chromatography
LC/MS/MS
liquid chromatography/tandem mass spectrometry
PKA
protein kinase A
PPI
protein–protein interaction
PSM
peptide spectrum match
PWMs
position weight matrices
TMT
tandem Mass Tag
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© 2021 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.