Asthma and lower airway diseaseTissue remodeling induced by hypersecreted epidermal growth factor and amphiregulin in the airway after an acute asthma attack
Section snippets
Study population
The study was approved by the Ethics Committee of Kanagawa Children's Medical Center and Nihon University Hospital, and all subjects provided written informed consent for participation, in accordance with the Helsinki Declaration of the World Medical Association. Fourteen children with asthma hospitalized for treatment of an acute attack, 13 stable outpatients before and after therapy with inhaled corticosteroids, 8 healthy controls, and 7 outpatients with respiratory tract infections without
Comparison of the levels of EGF and amphiregulin in the sputa of children with asthma collected during an asthma exacerbation and after recovery
Initially, we measured the concentrations of EGF, amphiregulin, TGF-α, and HB-EGF in the sputum samples of 6 patients with asthma collected during an exacerbation and after recovery. The concentration of HB-EGF in all the sputum samples was under the detection limit of the ELISA kit. The concentrations of TGF-α in the sputum samples were much lower than those of EGF and amphiregulin. The results are shown in this article's Table E1 in the Online Repository at www.jacionline.org. Therefore, we
Discussion
In this study, we measured, for the first time, the concentrations of EGF and amphiregulin in sputum samples from patients with asthma obtained during an acute attack and also during the recovery phase. We found that the sputum EGF levels in these patients with asthma increased during the acute attack and remained elevated during the recovery phase after the acute attack. In contrast, the sputum concentrations of amphiregulin and tryptase were only transiently elevated during the acute attack (
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2022, Molecular Aspects of MedicineCitation Excerpt :In response to these factors, activated myofibroblasts migrate to the sub-epithelium where they produce ECM proteins that contribute to basement membrane thickening, and ASM cells (ASMCs) undergo proliferation and hypertrophy leading to increased ASM mass (Flood-Page et al., 2003; Le Cras et al., 2011; Sidhu et al., 2010; Al Heialy et al., 2013; Perry et al., 2014; Sturrock et al., 2007). Due to the impaired tight junctions in the asthmatic epithelium, EGF produced at the basolateral side can activate the EGF receptor (EGFR) on the apical side of the epithelium promoting goblet cell metaplasia and increased mucous production (Lambrecht and Hammad, 2012; Enomoto et al., 2009). Disruption of epithelial barrier function and goblet cell metaplasia are also promoted by Th2 cytokines (Ahdieh et al., 2001; Kuperman et al., 2002).
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Supported in part by Grants-in-Aid for Scientific Research (C) programs of the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government (project nos. 18604009 and 20591195, awarded to Y.O.), the Nihon University Joint Research Grant for 2008 (awarded to Y.O), a grant from the National Institute of Biomedical Innovation (project no. ID05-24, awarded to H.S. and Y.O.), and the Matching Fund Subsidy for Private Universities from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government (to C.R).
Disclosure of potential conflict of interest: H. Saito has received research support from the National Institute of Biomedical Innovation. The rest of the authors have declared that they have no conflict of interest.