Developmental Cell
Volume 51, Issue 6, 16 December 2019, Pages 698-712.e8
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Article
Self-Organization of Mouse Stem Cells into an Extended Potential Blastoid

https://doi.org/10.1016/j.devcel.2019.11.014Get rights and content
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Highlights

  • Cell fate under extended potential conditions bifurcate to form EPI and PE-like lineages

  • EPSCs and TSCs self-organize into EPS-blastoids, resembling the late-stage blastocysts

  • EPS-blastoids undertake pre- to post-implantation transition in vitro

  • EPS-blastoids initiate implantation in vivo

Summary

Mammalian blastocysts comprise three distinct cell lineages essential for development beyond implantation: the pluripotent epiblast, which generates the future embryo, and surrounding it the extra-embryonic primitive endoderm and the trophectoderm tissues. Embryonic stem cells can reintegrate into embryogenesis but contribute primarily to epiblast lineages. Here, we show that mouse embryonic stem cells cultured under extended pluripotent conditions (EPSCs) can be partnered with trophoblast stem cells to self-organize into blastocyst-like structures with all three embryonic and extra-embryonic lineages. Morphogenetic and transcriptome profiling analyses reveal that these blastocyst-like structures show distinct embryonic-abembryonic axes and primitive endoderm differentiation and can initiate the transition from the pre- to post-implantation egg cylinder morphology in vitro.

Keywords

Extended pluripotency
Stem cells
Blastoid
Preimplantation
Embryo
Development
In Vitro
Trophoblast
Endoderm

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5

These authors contributed equally

6

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