Developmental Cell
Volume 39, Issue 1, 10 October 2016, Pages 44-60
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Article
Molecular Pathway of Microtubule Organization at the Golgi Apparatus

https://doi.org/10.1016/j.devcel.2016.08.009Get rights and content
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Highlights

  • CAMSAP2 stabilizes microtubule minus ends at the Golgi

  • A complex of AKAP450 and myomegalin recruits CAMSAP2-bound microtubules to the Golgi

  • A single Golgi can be maintained in the absence of centrosome and Golgi microtubules

  • Golgi microtubules facilitate cell reorientation and migration in 3D matrix

Summary

The Golgi apparatus controls the formation of non-centrosomal microtubule arrays important for Golgi organization, polarized transport, cell motility, and cell differentiation. Here, we show that CAMSAP2 stabilizes and attaches microtubule minus ends to the Golgi through a complex of AKAP450 and myomegalin. CLASPs stabilize CAMSAP2-decorated microtubules but are not required for their Golgi tethering. AKAP450 is also essential for Golgi microtubule nucleation, and myomegalin and CDK5RAP2 but not CAMSAP2 contribute to this function. In the absence of centrosomes, AKAP450- and CAMSAP2-dependent pathways of microtubule minus-end organization become dominant, and the presence of at least one of them is needed to maintain microtubule density. Strikingly, a compact Golgi can be assembled in the absence of both centrosomal and Golgi microtubules. However, CAMSAP2- and AKAP450-dependent Golgi microtubules facilitate Golgi reorientation and cell invasion in a 3D matrix. We propose that Golgi-anchored microtubules are important for polarized cell movement but not for coalescence of Golgi membranes.

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