Developmental Cell
Volume 12, Issue 1, January 2007, Pages 17-30
Journal home page for Developmental Cell

Article
Priming of Centromere for CENP-A Recruitment by Human hMis18α, hMis18β, and M18BP1

https://doi.org/10.1016/j.devcel.2006.11.002Get rights and content
Under an Elsevier user license
open archive

Summary

The centromere is the chromosomal site that joins to microtubules during mitosis for proper segregation. Determining the location of a centromere-specific histone H3 called CENP-A at the centromere is vital for understanding centromere structure and function. Here, we report the identification of three human proteins essential for centromere/kinetochore structure and function, hMis18α, hMis18β, and M18BP1, the complex of which is accumulated specifically at the telophase-G1 centromere. We provide evidence that such centromeric localization of hMis18 is essential for the subsequent recruitment of de novo-synthesized CENP-A. If any of the three is knocked down by RNAi, centromere recruitment of newly synthesized CENP-A is rapidly abolished, followed by defects such as misaligned chromosomes, anaphase missegregation, and interphase micronuclei. Tricostatin A, an inhibitor to histone deacetylase, suppresses the loss of CENP-A recruitment to centromeres in hMis18α RNAi cells. Telophase centromere chromatin may be primed or licensed by the hMis18 complex and RbAp46/48 to recruit CENP-A through regulating the acetylation status in the centromere.

CELLBIO
CELLCYCLE

Cited by (0)

4

Present address: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.

5

Present address: Faculty of Advanced Life Science, Hokkaido University, Kita-21, Nishi-11, Sapporo, Hokkaido 001-0021, Japan.