Cell Metabolism
Volume 12, Issue 1, 7 July 2010, Pages 30-41
Journal home page for Cell Metabolism

Article
EAK-7 Controls Development and Life Span by Regulating Nuclear DAF-16/FoxO Activity

https://doi.org/10.1016/j.cmet.2010.05.004Get rights and content
Under an Elsevier user license
open archive

Summary

FoxO transcription factors control development and longevity in diverse species. Although FoxO regulation via changes in its subcellular localization is well established, little is known about how FoxO activity is regulated in the nucleus. Here, we show that the conserved C. elegans protein EAK-7 acts in parallel to the serine/threonine kinase AKT-1 to inhibit the FoxO transcription factor DAF-16. Loss of EAK-7 activity promotes diapause and longevity in a DAF-16/FoxO-dependent manner. Whereas akt-1 mutation activates DAF-16/FoxO by promoting its translocation from the cytoplasm to the nucleus, eak-7 mutation increases nuclear DAF-16/FoxO activity without influencing DAF-16/FoxO subcellular localization. Thus, EAK-7 and AKT-1 inhibit DAF-16/FoxO activity via distinct mechanisms. Our results implicate EAK-7 as a FoxO regulator and highlight the biological impact of a regulatory pathway that governs the activity of nuclear FoxO without altering its subcellular location.

Highlights

eak-7 emerged from a C. elegans screen for enhancers of the akt-1 mutant phenotype ► EAK-7 controls larval development and adult life span by inhibiting DAF-16/FoxO ► EAK-7 and AKT-1 inhibit DAF-16/FoxO via distinct mechanisms ► EAK-7 inhibits nuclear DAF-16/FoxO without promoting its cytoplasmic translocation

SIGNALING
DEVBIO

Cited by (0)