Cell Reports
Volume 23, Issue 11, 12 June 2018, Pages 3275-3285
Journal home page for Cell Reports

Article
Inhibition of Microprocessor Function during the Activation of the Type I Interferon Response

https://doi.org/10.1016/j.celrep.2018.05.049Get rights and content
Under a Creative Commons license
open access

Highlights

  • Antiviral response transiently impairs Microprocessor function

  • Microprocessor binding and processing efficiency is regulated by IFN response

  • Regulation of Microprocessor activity is essential for robust IFN expression

Summary

Type I interferons (IFNs) are central components of the antiviral response. Most cell types respond to viral infections by secreting IFNs, but the mechanisms that regulate correct expression of these cytokines are not completely understood. Here, we show that activation of the type I IFN response regulates the expression of miRNAs in a post-transcriptional manner. Activation of IFN expression alters the binding of the Microprocessor complex to pri-miRNAs, reducing its processing rate and thus leading to decreased levels of a subset of mature miRNAs in an IRF3-dependent manner. The rescue of Microprocessor function during the antiviral response downregulates the levels of IFN-β and IFN-stimulated genes. All these findings support a model by which the inhibition of Microprocessor activity is an essential step to induce a robust type I IFN response in mammalian cells.

Keywords

microRNAs
microprocessor
DGCR8
Drosha
type I interferon
dsRNA
antiviral

Cited by (0)

2

Lead Contact