Cell Reports
Volume 6, Issue 3, 13 February 2014, Pages 445-454
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Recycling Endosome Tubule Morphogenesis from Sorting Endosomes Requires the Kinesin Motor KIF13A

https://doi.org/10.1016/j.celrep.2014.01.002Get rights and content
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Highlights

  • The kinesin-3 KIF13A associates with endosomal tubules

  • KIF13A controls the morphogenesis of recycling tubules from sorting endosomes

  • KIF13A interacts with the GTP-bound form of RAB11

  • KIF13A and RAB11 cooperate for generation of endosomal tubules

Summary

Early endosomes consist of vacuolar sorting and tubular recycling domains that segregate components fated for degradation in lysosomes or reuse by recycling to the plasma membrane or Golgi. The tubular transport intermediates that constitute recycling endosomes function in cell polarity, migration, and cytokinesis. Endosomal tubulation and fission require both actin and intact microtubules, but although factors that stabilize recycling endosomal tubules have been identified, those required for tubule generation from vacuolar sorting endosomes (SEs) remain unknown. We show that the microtubule motor KIF13A associates with recycling endosome tubules and controls their morphogenesis. Interfering with KIF13A function impairs the formation of endosomal tubules from SEs with consequent defects in endosome homeostasis and cargo recycling. Moreover, KIF13A interacts and cooperates with RAB11 to generate endosomal tubules. Our data illustrate how a microtubule motor couples early endosome morphogenesis to its motility and function.

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Present address: Membrane Dynamics and Intracellular Trafficking, Institut Jacques Monod, UMR 7592, CNRS, Université Paris Diderot, Sorbonne Paris Cité, Paris 75013, France