Sensory Neuron Diversity in the Inner Ear Is Shaped by Activity

Highlights

• Three molecular subtypes of type I spiral ganglion neurons (SGNs) identified

• Additional molecular variations exist across subtypes and along the tonotopic axis

• Subtype identities emerge around birth and are refined over postnatal stages

• Disruption of hair-cell-driven activity leads to aberrant SGN subtype diversity

Summary

In the auditory system, type I spiral ganglion neurons (SGNs) convey complex acoustic information from inner hair cells (IHCs) to the brainstem. Although SGNs exhibit variation in physiological and anatomical properties, it is unclear which features are endogenous and which reflect input from synaptic partners. Using single-cell RNA sequencing, we derived a molecular classification of mouse type I SGNs comprising three subtypes that express unique combinations of Ca²⁺ binding proteins, ion channel regulators, guidance molecules, and transcription factors. Based on connectivity and susceptibility to age-related loss, these subtypes correspond to those defined physiologically. Additional intrinsic differences among subtypes and across the tonotopic axis highlight an unexpectedly active role for SGNs in auditory processing. SGN identities emerge postnatally and are disrupted in a mouse model of deafness that lacks IHC-driven activity. These results elucidate the range, nature, and origins of SGN diversity, with implications for treatment of congenital deafness.