Prolonged blockade of nitric oxide synthesis in gravid rats produces sustained hypertension, proteinuria, thrombocytopenia, and intrauterine growth retardation*,**,*,★★
Section snippets
Reagents
Ketamine hydrochloride (Ketanest) and pentobarbital sodium (Nembutal) were purchased from Parke-Davis (München, Germany) and from Veterinary Laboratories (Lenexa, Kan.), respectively. L-NAME and all other chemicals used in this study were obtained from Sigma (St. Louis).
Experimental protocol
Wistar-derived, timed-pregnant and virgin rats (LATI, Gödöllö, Hungary) were housed individually in standard metabolic cages, allowing for the collection of urine and recording of food and water consumption throughout the study.
RESULTS
The mean body weight, food and water intake, and urine volume before (control) and on the third day of infusion (treated) are summarized in Table I. In spite of similar food intake, the body weight of L-NAME–treated mothers was lower than that of saline solution–treated controls, probably reflecting the significant growth retardation of conceptuses. Interestingly, L-NAME infusion significantly increased urine volume in both pregnant and virgin groups compared with corresponding saline
COMMENT
A previous study3 has demonstrated that an 8-hour infusion of L-NAME brought about a marked hypertension in chronically instrumented conscious rats and abolished the refractoriness to angiotensin II, vasopressin, and norepinephrine, a hallmark of hemodynamic changes surrounding preeclampsia.14, 15 By means of the same animal model the current investigation was undertaken to evaluate the long-term effects of controlled, continuous intravenous infusion of L-NAME on blood pressure, fetal
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From the Departments of Obstetrics and Gynecologya and Pharmacological and Physiological Science,b St. Louis University School of Medicine, the Institute of Pathophysiology, Semmelweis Medical University,c and the Department of Pathology, Veszprém County Hospital.d
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Supported in part by the Hungarian Kidney Foundation.
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Reprint requests: Frank Hertelendy, PhD, DSc, Department of Obstetrics and Gynecology, St. Louis University Health Sciences Center, 3635 Vista Ave. at Grand Blvd., P.O. Box 15250, St. Louis, MO 63110-0250.
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