Elsevier

Molecular Immunology

Volume 30, Issue 8, June 1993, Pages 755-764
Molecular Immunology

The cysteine residues in the cytoplasmic tail of CD8 α are required for its coreceptor function

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Abstract

The cytoplasmic segment of the CD8 α polypeptide includes both a cysteine-containing motif that is required for its association with the tyrosine kinase p56lck, and two serine residues which are likely to be phosphorylated and involved in inside-out signaling phenomena. To determine the relative importance of these residues for CD8 function, a mouse T cell hybridoma expressing a T cell receptor specific for the class I major histocompatibility product H-2Kb was transfected with a set of CD8 α chain genes encoding polypeptides in which the cytoplasmic cysteine or serine residues were substituted with alanine. When challenged with Kb-transfected L cells, T cell transfectants expressing CD8 αβ or CD8 αα dimers with substituted cytoplasmic serine residues responded nearly as well as wild-type CD8 transfectants. In marked contrast, the CD8 α polypeptides bearing substitutions of both cytoplasmic cysteine residues were totally impaired in their ability to complement the co-expressed T cell receptor.

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