Elsevier

Plasmid

Volume 24, Issue 1, July 1990, Pages 74-80
Plasmid

Short communication
Nucleotide sequence of therep gene of staphylococcal plasmid pCW7

https://doi.org/10.1016/0147-619X(90)90027-AGet rights and content

Abstract

Previous heteroduplex mapping studies showed that staphylococcal plasmid pCW7 belongs to the pT181 family of small antibiotic resistance plasmids which replicate by a rolling-circle mechanism. Replication in each case is initiated at the plasmid origin (ori) by a plasmid-encoded protein, Rep, which makes a sequence-specific single-stranded nick to form a covalent Rep-ori replication intermediate. A comparison of sequencing results for therepN gene of pCW7 with data for the products of five other homologousrep genes allows a prediction to be made of the segments of the primary structure of Rep which are likely to be responsible for plasmid-specific recognition of theori region by each Rep protein.

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    This family is also included by small bacterial plasmids, such as the Bacillus subtilis plasmid, pUH1 (Hara et al., 1991), and the mobilizable vancomycin-resistance plasmid pDT1 found in Enterococcus faecium (Todokoro et al., 2006), replicated by a rolling-circle mechanism. The Rep_trans family is represented by RepN, which likely functions to recognize the ori region of the staphylococcal plasmid, pCW7 (Balson and Shaw, 1990). This family is also included by other small resistance plasmids, such as pNS1 (Aoki et al., 1993) and pUB112 (probably an IncFII plasmid) (Ehret and Matzura, 1988), which are staphylococcal tetracycline-resistant and chloramphenicol-resistant plasmids, respectively.

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Sequence data from the article have been deposited with the EMBL/GenBank Data Libraries under Accession No. J03323.

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