Elsevier

Brain Research

Volume 635, Issues 1–2, 28 January 1994, Pages 307-311
Brain Research

Chondroitin sulfate proteoglycan-immunoreactivity of lectin-labeled perineuronal nets around parvalbumin-containing neurons

https://doi.org/10.1016/0006-8993(94)91452-4Get rights and content

Abstract

Perineuronal nets represent highly specialized glial and glia-associated structures. In this study, a triple fluorescence labeling of chondroitin sulfate proteoglycan-immunoreactive (CSPG-ir) andN-acetylgalactosamine (GalNac)-specific plant lectinWisteria floribunda agglutinin (WFA) binding net components as well as parvalbumin-immunoreactivity (-ir) was performed. It was shown in the rat cortex, that the same nets frequently surrounding parvalbumin-ir neurons are stained by CSPG-ir as well as by the lectin binding method.

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      These proteins bind to both the HA backbone and CSPGs to stabilize PNNs [49,56–58]. PNNs are most commonly identified by staining them with the plant lectin, Wisteria floribunda agglutinin (WFA; Fig. 2), which is thought to bind to terminal N-acetylgalactosamines beta 1 residues of glycoproteins [59], most likely on aggrecan; other methods for staining PNNs have also been used but probably bind to the same residues [59–62]. The HA synthesizing enzymes, HAS1, HAS2, and HAS3, are found in neurons with PNNs [45].

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