Metabolism of lysine in rat liver

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Abstract

It was shown previously that lysine was degraded in the mitochondria in the presence of α-ketoglutarate and, under these conditions, saccharopine accumulated (1). The obligatory requirement of α-ketoglutarate for lysine breakdown as well as for saccharopine formation suggest that lysine is metabolized, in mammalian liver, by a reversal of reaction sequence described for its biosynthesis in yeast (2–6).

The present report describes that saccharopine is further metabolized to α-aminoadipic acid in the mitochondria and that NAD rather than NADP is required in this reaction.

References (8)

  • K. Higashino et al.

    Biochem. Biophys. Res. Comm

    (1965)
  • M.H. Kuo et al.

    J. Biol. Chem

    (1964)
  • J.S. Trupin et al.

    J. Biol. Chem

    (1965)
  • E.E. Jones et al.

    J. Biol. Chem

    (1965)
There are more references available in the full text version of this article.

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