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Shotgun Analysis of the Secretome of Fusarium graminearum

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Abstract

Fusarium head blight, caused predominately by Fusarium graminearum, is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. To characterize the profile of proteins secreted by F. graminearum, the extracellular proteins were collectively obtained from F. graminearum culture supernatants and evaluated using one-dimensional SDS-PAGE and liquid chromatography-tandem mass spectrometry. A total of 87 proteins have been identified, of which 63 were predicted as secretory proteins including those with known functions. Meanwhile, 20 proteins that are not homologous to genomic sequences with known functions have also been detected. Some of the identified proteins are possible virulence factors and may play extracellular roles during F. graminearum infection. This study provides a valuable dataset of F. graminearum extracellular proteins, and a better understanding of the virulence mechanisms of the pathogen.

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Acknowledgments

This work was funded by the Natural Science Foundation of China (Grant No. 31070573, 31071405, 31171553) and the National Basic Research Program of China (973) (Grant No. 2009CB118301).

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Correspondence to Xian-Ling Ji or Ling-Rang Kong.

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Xian-Ling Ji, Mei Yan contributed equally to this study.

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Ji, XL., Yan, M., Yang, ZD. et al. Shotgun Analysis of the Secretome of Fusarium graminearum . Indian J Microbiol 53, 400–409 (2013). https://doi.org/10.1007/s12088-013-0392-1

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  • DOI: https://doi.org/10.1007/s12088-013-0392-1

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