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MiR-124 Promotes Newborn Olfactory Bulb Neuron Dendritic Morphogenesis and Spine Density

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Abstract

Using microarray analysis, we detected microRNA-124 (miR-124) to be abundantly expressed in the olfactory bulb (OB). miR-124 regulates adult neurogenesis in the subventricular zone (SVZ). However, much less is known about its role in newborn OB neurons. Here, using both gain-of-function and loss-of-function approaches, we demonstrate that brain-specific miR-124 affects dendritic morphogenesis and spine density in newborn OB neurons. Functional Annotation Clustering of miR-124 targets was enriched in “cell morphogenesis involved in neuron differentiation.”

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Acknowledgements

We are grateful to the Core Facilities of Zhejiang University Institute of Neuroscience for the technical assistance.

Author’s Contributions

Guifa Li carried out the sample collection, participated in the array analysis, and drafted the manuscript. Shucai Ling participated in the cell culture and drafted the manuscript. All authors have read and approved the final manuscript.

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Authors and Affiliations

  1. Department of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China

    Guifa Li & Shucai Ling

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  1. Guifa Li
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  2. Shucai Ling
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Correspondence to Shucai Ling.

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All experiments were approved by the Animal Advisory Committee at Zhejiang University and conducted in accordance with the US National Institutes of Health Guidelines for the Care and Use of Laboratory Animals.

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The authors declare that they have no conflict of interest.

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Li, G., Ling, S. MiR-124 Promotes Newborn Olfactory Bulb Neuron Dendritic Morphogenesis and Spine Density. J Mol Neurosci 61, 159–168 (2017). https://doi.org/10.1007/s12031-016-0873-x

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  • DOI: https://doi.org/10.1007/s12031-016-0873-x

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