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High glucose upregulates pantothenate kinase 4 (PanK4) and thus affects M2-type pyruvate kinase (Pkm2)

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Abstract

A new Rattus norvegicus PanK gene was isolated by mRNA differential display from high concentration glucose-stimulated rat, which encodes a human PanK4-like protein with KOG2201 and KOG4584 domain. Proteins that interact with rat PanK4 were identified by the application of the yeast two-hybrid system. One of the components, Pkm2, was found to be associated with rat PanK4 and its two domains under both in vitro and in vivo conditions. Immunofluorescence staining and confocal scanning experiments showed that PanK4 could transiently co-express with Pkm2 in the cytoplasm of HeLa cell and HEK293T cell. These findings suggest that PanK4 interacts with Pkm2 and thereby may modulate the glucose metabolism through regulating the activity of Pkm2.

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Abbreviations

PanK:

Pantothenate kinase

Pkm2:

M2-type pyruvate kinase

aa:

amino acid residues

GST:

glutathione-S-transferase

PBS:

phosphate-buffered saline

HEK:

human embryonic kidney

MoAb:

monoclonal antibody

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Author notes

  1. Yunfeng Li

    Present address: Department of Biological Chemistry, University of California, Irvine, CA, 92697, USA

Authors and Affiliations

  1. National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences, 5# Dong Dan San Tiao, Beijing, P.R. China, 100005

    Yunfeng Li, Yongsheng Chang, Lifeng Zhang, Qiping Feng, Zhuo Liu, Yongwei Zhang, Jin Zuo, Yan Meng & Fude Fang

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  1. Yunfeng Li
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  2. Yongsheng Chang
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  3. Lifeng Zhang
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  4. Qiping Feng
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  5. Zhuo Liu
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  6. Yongwei Zhang
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  7. Jin Zuo
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Li, Y., Chang, Y., Zhang, L. et al. High glucose upregulates pantothenate kinase 4 (PanK4) and thus affects M2-type pyruvate kinase (Pkm2). Mol Cell Biochem 277, 117–125 (2005). https://doi.org/10.1007/s11010-005-5535-1

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  • DOI: https://doi.org/10.1007/s11010-005-5535-1

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