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Shaker-like potassium channels in Populus, regulated by the CBL–CIPK signal transduction pathway, increase tolerance to low-K+ stress

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Abstract

Shaker-like potassium channels in plants play an important role in potassium absorption and transport. Here, we characterized 11 genes encoding shaker-like channels from Populus trichocarpa. Furthermore, two homologs from this family were isolated from Populus euphratica and named PeKC1 and PeKC2. Subcellular localization analysis of them in Nicotiana benthamiana revealed that they are located in the cell membrane. Yeast two-hybrid assays showed that they not only interacted strongly with PeCIPK24, a homolog of AtCIPK23, but also interacted with several other CIPK members, including PeCIPK10 and PeCIPK17. To further analyze their function, we over-expressed PeKC1 or PeKC2 in akt1 mutant, the results show that the transgenic plant can recover the mutant phonotype sensitive to low-K+ stress. This means PeKC1 or PeKC2 can complement the function of AKT1 in akt1 mutant, involved in the CBL1-CIPK23 signal transduction pathway and play an important role under low-K+ stress.

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Acknowledgments

This research was supported by the Hi-Tech Research and Development Program of China (2007AA10Z106), National Natural Science Foundation of China (30730077, 30972339), Program for New Century Excellent Talents in University of China (NCET-07-0083), “948” Project of State Forestry Administration of China (2007-4-01) and the 111 project (B08005). Also we thank Sheng Luan in UC Berkeley for providing akt1 mutant to us.

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Correspondence to Weilun Yin or Xinli Xia.

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Communicated by E. Guiderdoni.

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Zhang, H., Yin, W. & Xia, X. Shaker-like potassium channels in Populus, regulated by the CBL–CIPK signal transduction pathway, increase tolerance to low-K+ stress. Plant Cell Rep 29, 1007–1012 (2010). https://doi.org/10.1007/s00299-010-0886-9

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