Skip to main content
SpringerLink
Log in

Multiple sclerosis patients have peripheral blood CD45RO+ B cells and increased intestinal permeability

  • Intestinal Disorders, Inflammatory Bowel Disease, And Irritable Bowel Syndrome
  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

Increased intestinal permeability and the CD45RO isoform expression of the leukocyte common antigen on peripheral blood CD20+ B cells are found in Crohn's disease. Others have observed that multiple sclerosis (MS) patients may have an increased risk of coacquisition of Crohn's disease. The aim of this study was to identify an association between these diseases using peripheral blood CD45 isoform expression and intestinal permeability in MS. Lactulose/mannitol permeability and peripheral blood CD20+ B cell CD45RO expression were defined in healthy controls, MS patients, and patients coincidentally affected by MS and Crohn's or MS and ulcerative colitis (UC). Five of 20 MS patients had increased intestinal permeability, a finding not previously reported. High levels of CD45RO were found on circulating CD20+ B cells from patients with MS. This has not been reported previously in MS and is found in very few other conditions. Eight patients with coincident MS and Crohn's disease or MS and UC were studied. Coincident MS and UC patients expressed CD45RO on CD20+ B cells, a finding not identified in UC patients alone. A subgroup of MS patients has increased intestinal permeability. These patients express CD45RO CD20+ B cells, also found in Crohn's disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Pinchbeck BR, Kirdeikis J, Thomson ABR: Inflammatory bowel disease in northern Alberta. An epidemiologic study. J Clin Gastroenterol 10:505–515, 1988

    PubMed  Google Scholar 

  2. Kurtzke JF: Epidemiology of multiple sclerosis.In Multiple Sclerosis, JF Hallpike CWM Adams, WW Tourtelotte (eds). Baltimore, Williams & Wilkins, 1983, pp 47–95

    Google Scholar 

  3. Demaine AG: The molecular biology of autoimmune disease. Immunol Today 10:357–361, 1989

    PubMed  Google Scholar 

  4. Wucherpfennig KW, Weiner HL, Hafler DA: T-cell recognition of myelin basic protein. Immunol Today 12:277–282, 1991

    PubMed  Google Scholar 

  5. Minuk GY, Lewkonia RM: Possible familial association of multiple sclerosis and inflammatory bowel disease. N Engl J Med 314(9):586, 1986

    PubMed  Google Scholar 

  6. Purrmann J, Arendt G, Cleveland S, Borchard F, Furst N, Gemsa R, Bertrams J, Hengels KJ: Association of Crohn's disease and multiple sclerosis. Is there a common background? J Clin Gastroenterol 14(1):43–46, 1991

    Google Scholar 

  7. Sadovnick AD, Paty DW, Yannakoulias G: Concurrence of multiple sclerosis and inflammatory bowel disease. N Engl J Med 321:762–763, 1989

    Google Scholar 

  8. Kitchin LI, Knobler RL, Friedman LS: Crohn's disease in a patient with multiple sclerosis. J Clin Gastroenterol 13:331–334, 1991

    PubMed  Google Scholar 

  9. Agranoff D, Schon F: Are focal white matter lesions in patients with inflammatory bowel disease linked to multiple sclerosis? Lancet 346(8968):190–191, 1995

    Google Scholar 

  10. Geissler A, Andus T, Roth M, Kullmann F, Caesar I, Held P, Gross V, Feuerbach S, Scholmerich J: Focal white matter lesions in brain of patients with inflammatory bowel disease. Lancet 345(8954):897–898, 1995

    PubMed  Google Scholar 

  11. Warren KG, Katz I: Purification of primary antibodies of the myelin basic protein antibody cascade from multiple sclerosis patients. Immunoreactivity studies with homologous and heterologous antigens. Clin Invest Med 15:18–29, 1992

    PubMed  Google Scholar 

  12. Mayer L, Eisenhardt D: Lack of induction of suppressor T-cells by intestinal epithelial cells from patients with inflammatory bowel disease. J Clin Invest 86:1255–1260, 1990

    PubMed  Google Scholar 

  13. Sun JB, Olsson T, Wang WZ, Xiao BG, Kostulas V, Fredrikson S, Ekre HP, Link H: Autoreactive T and B-cells responding to myelin proteolipid protein in multiple sclerosis and controls. Eur J Immunol 21:1461–1468, 1991

    PubMed  Google Scholar 

  14. Mix E, Olsson T, Correale J, Baig S, Kostulas V, Olsson O, Link H: B-cells expressing CD5 are increased in cerebrospinal fluid of patients with multiple sclerosis. Clin Exp Immunol 79:21–27, 1990

    PubMed  Google Scholar 

  15. Martino G, Olsson T, Fredrikson S, Hojeberg B, Kostulas V, Grimaldi LM, Link H: Cells producing antibodies specific for myelin basic protein region 70–89 are predominant in cerebrospinal fluid from patients with multiple sclerosis. Eur J Immunol 21:2971–2976, 1991

    PubMed  Google Scholar 

  16. Michielsens B, Walravens M, Vermylen J, Carton H: Diagnostic significance of antinuclear antibodies in neurologic patients. Acta Neurol Scand 84:102–106, 1991

    PubMed  Google Scholar 

  17. Carr RI, Weldon P, Sadi D, Murray TJ: Autoantibodies toβ-endorphin in patients with multiple sclerosis (MS). Clin Invest Med 15 (Suppl):A-16, 1992

    Google Scholar 

  18. MacDermott RP, Nash GS, Aver IO, Shlien R, Kewis BS, Madassery J, Nahn MH: Alterations in serum immunoglobulin G subclasses in patients with ulcerative colitis and Crohn's disease. Gastroenterology 96:764–768, 1989

    PubMed  Google Scholar 

  19. Yacyshyn BR, Pilarski LM: Expression of CD45RO on circulating CD19+ B-cells in Crohn's disease. Gut 34:1698–1704, 1993

    PubMed  Google Scholar 

  20. Pilarski LM, Jensen GS: Expression of CD45 isoforms (leukocyte common antigen) and adhesion molecules during normal and abnormal human B-lymphocyte development.In Advances in Molecular and Cellular Immunology, Vol 1B, B Singh (ed). Greenwich, CT, JAI, 1993, pp 75–118

    Google Scholar 

  21. Thomas ML: The leukocyte common antigen family. Annu Rev Immunol 7:339–369, 1989

    PubMed  Google Scholar 

  22. Pilarski LM, Gillitzer R, Zola H, Shortman K, Scollay R: Definition of the thymic generative lineage by selective expression of high molecular weight isoforms of CD45 (T200). Eur J Immunol 19:589–597, 1989

    PubMed  Google Scholar 

  23. Jensen GS, Poppema S, Mant MJ, Pilarski LM: Transition in CD45 isoform expression during differentiation of normal and abnormal B-cells. Int Immunol 1:229–236, 1989

    PubMed  Google Scholar 

  24. Pilarski LM, Jensen GS: Monoclonal circulating B-cells in multiple myeloma. A continuously differentiating possibly invasive population as defined by expression of CD45 isoforms and adhesion molecules. Hematol/Oncol Clin North Am 6:297–322, 1992

    Google Scholar 

  25. Yacyshyn BR: Activated CD19 B-cell lamina propria lymphocytes in ulcerative colitis. Immunol Cell Biol 71:265–274, 1993

    PubMed  Google Scholar 

  26. Yacyshyn BR, Meddings JB: CD45RO expression on circulating CD19+ B-cells in Crohn's disease correlates with intestinal permeability. Gastroenterology 108:132–137, 1995

    PubMed  Google Scholar 

  27. Poser CM, Paty DW, Scheinberg L, McDonald WI, Davis FA, Ebers GC, Johnson KP, Sibley WA, Silberberg DH, Tourtellotte WW: New diagnostic criteria for multiple sclerosis: Guidelines for research protocols. Ann Neurol 13:227–231, 1983

    PubMed  Google Scholar 

  28. Harvey J, Jones DB, Wright DH: Leukocyte common antigen expression on T-cells in normal and inflamed human gut. Immunology 68:13–17, 1989

    PubMed  Google Scholar 

  29. Rodriguez M, Scheithauer BW, Forbes G, Kelly PJ: Oligodendrocyte injury is an early event in lesions of multiple sclerosis. Mayo Clin Proc 68:627–636, 1993

    PubMed  Google Scholar 

  30. Yacyshyn BR, Lazarovits A, Tsai V, Matejko K: Crohn's disease, ulcerative colitis and normal intestinal lymphocytes express integrins in dissimilar patterns. Gastroenterology 107:1364–1371, 1994

    PubMed  Google Scholar 

  31. Sobel RA, Mitchell ME, Fondren G: Intercellular adhesion molecule-1 (ICAM-1) in cellular immune reactions in the human central nervous system. Am J Pathol 136(6):1309–1316, 1990

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. From the Department of Medicine, Division of Gastroenterology, University of Alberta, Edmonton, Alberta, Canada

    Bruce Yacyshyn MD (FRCP(C), Jon Meddings MD (FRCP(C), Daniel Sadowski MD (FRCP(C) & Mary Beth Bowen-Yacyshyn PhD

  2. Department of Medicine, Division of Gastroenterology, University of Calgary, Calgary, Alberta, Canada

    Bruce Yacyshyn MD (FRCP(C), Jon Meddings MD (FRCP(C), Daniel Sadowski MD (FRCP(C) & Mary Beth Bowen-Yacyshyn PhD

Authors
  1. Bruce Yacyshyn MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jon Meddings MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Daniel Sadowski MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mary Beth Bowen-Yacyshyn PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yacyshyn, B., Meddings, J., Sadowski, D. et al. Multiple sclerosis patients have peripheral blood CD45RO+ B cells and increased intestinal permeability. Digest Dis Sci 41, 2493–2498 (1996). https://doi.org/10.1007/BF02100148

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02100148

Key words

Search

Navigation