Summary
The distribution of tubulin in brain tissue has been investigated by light and electron microscopic immunohistochemistry using an antiserum raised against electrophoretically purified microtubule-derived tubulin. The specificity of the staining is indicated by the lack of staining when non-immune serum is substituted for anti-tubulin serum and the quite different staining obtained with antisera against antigens other than tubulin. The anti-tubulin immunohistochemistry reveals tubulin antigen in microtubules and in the postsynaptic junctional lattice of most synapses. No antigen has been detected in either the synaptic cleft or in the axon terminal. This distribution confirms previous biochemical identification of tubulin as a major postsynaptic junctional component, but suggests that it is not common to all synaptic junctions.
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Banker, G., Churchill, L. andCotman, C. W. (1974) Proteins of the postsynaptic density.Journal of Cell Biology 63, 456–65.
Berlin, R. D., Oliver, J. M., Ukena, T. E. andYin, H. H. (1974) Control of cell surface topography.Nature 247, 45–6.
Blitz, A. L. andFine, R. E. (1974) Muscle-like contractile proteins and tubulin in synaptosomes.Proceedings of the National Academy of Sciences (U.S.A.) 71, 4472–6.
Borisy, G. G. andTaylor, E. W. (1969) The mechanism of action of colchicine. Binding of colchicine-3H to cellular protein.Journal of Cell Biology 34, 525–33.
Cotman, C. W., Banker, G., Churchill, L. andTaylor, D. (1974) Isolation of postsynaptic densities from rat brain.Journal of Cell Biology 63, 441–55.
Feit, H. andBarondes, S. (1970) Colchicine-binding activity in paniculate fractions from mouse brain.Journal of Neurochemistry 17, 1355–63.
Feit, H., Dutton, G. R., Barondes, S. H. andShelanski, M. L. (1971) Microtubule protein: Identification and transport to nerve endings.Journal of Cell Biology 51, 138–47.
Gray, E. G. (1975) Presynaptic microtubules and associated synaptic vesicles.Proceedings of the Royal Society of London, Series B, in press.
Kadota, T., Kadota, K. AndGray, E. G. (1975) Coated vesicles, particle/chain material and tubulin brain synaptosomes.Journal of Cell Biology, in press.
Lagnado, J. R., Lyons, C. andWickremasinghe, G. (1971) The subcellular distribution of colchicine-binding protein (‘microtubule protein’) in rat brain.Febs Letters,15, 254.
Matus, A. I. andMughal, S. (1975) Immunohistochemical localisation of S-100 protein in the brain.Nature, in press.
Matus, A. I. andWalters, B. B. (1975) Ultrastructure of the synaptic junctional lattice isolated from mammalian brain.Journal of Neurocytology 4, 369–75.
Matus, A. I., Walters, B. B. andJones, D. H. (1975) Junctional ultrastructure in isolated synaptic membranes.Journal of Neurocytology 4, 357–67.
Redburn, D. A. andCotman, C. W. (1974) Calcium-dependent release of (14C) GABA from vinblas- and colchicine treated synaptosomes.Brain Research 73, 550–7.
Shelanski, M. L., Gaskin, F. andCantor, C. R. (1973) Microtubule assembly in the absence of added nucleotides.Proceedings of the National Academy of Sciences (U.S.A.) 70, 765–8.
Walters, B. B. andMatus, A. I. (1974) Ultrastructural organisation in isolated synaptic densities.Journal of Anatomy (London) 119, 415.
Walters, B. B. andMatus, A. I. (1975a) Proteins of the synaptic junction.Biochemical Society Transactions 3, 109–12.
Walters, B. B. andMatus, A. I. (1975b) Tubulin in the postsynaptic junctional lattice.Nature, in press.
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Matus, A.I., Walters, B.B. & Mughal, S. Immunohistochemical demonstration of tubulin associated with microtubules and synaptic junctions in mammalian brain. J Neurocytol 4, 733–744 (1975). https://doi.org/10.1007/BF01181633
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DOI: https://doi.org/10.1007/BF01181633