Abstract
Histopathology is a defining endpoint in mouse models of experimental gastritis and gastric adenocarcinoma. Presented here is an overview of the histology of gastritis and gastric cancer in mice experimentally infected with Helicobacter pylori or H. felis. A modular histopathologic scoring scheme is provided that incorporates relevant disease-associated changes. Whereas the guide uses Helicobacter infection as the prototype challenge, features may be applied to chemical and genetically engineered mouse models of stomach cancer as well. Specific criteria included in the combined gastric histologic activity index (HAI) include inflammation, epithelial defects, oxyntic atrophy, hyperplasia, pseudopyloric metaplasia, and dysplasia or neoplasia. Representative photomicrographs accompany descriptions for each lesion grade. Differentiation of genuine tumor invasion from pseudoinvasion is highlighted. A brief comparison of normal rodent versus human stomach anatomy and physiology is accompanied by an introduction to mouse-specific lesions including mucous metaplasia and eosinophilic droplets (hyalinosis). In conjunction with qualified pathology support, this guide is intended to assist research scientists, postdoctoral fellows, graduate students, and medical professionals from affiliated disciplines in the interpretation and histologic grading of chronic gastritis and gastric carcinoma in mouse models.
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Correa P (1985) Mechanisms of gastric carcinogenesis. In: Joossens JV, Hill MJ, Geboers J (eds) Diet and human carcinogenesis. Elsevier, Amsterdam, The Netherlands), pp 109–115
Wroblewski LE, Peek RM Jr, Wilson KT (2010) Helicobacter pylori and gastric cancer: factors that modulate disease risk. Clin Microbiol Rev 23:713–739
Rogers AB, Fox JG (2004) Inflammation and Cancer I. Rodent models of infectious gastrointestinal and liver cancer. Am J Physiol Gastrointest Liver Physiol 286:G361–G366
Rogers AB, Fox JG (2009) Animal models of gastric carcinoma. In: Giraud AS, Fox JG, Wang TC (eds) The biology of gastric cancers. Springer, New York, pp 323–360
Rogers AB, Houghton J (2009) Helicobacter-based mouse models of digestive system carcinogenesis. Methods Mol Biol 511:267–295
Houghton J, Stoicov C, Nomura S et al (2004) Gastric cancer originating from bone marrow-derived cells. Science 306:1568–1571
Correa P (1995) Helicobacter pylori and gastric carcinogenesis. Am J Surg Pathol 19(Suppl 1):S37–S43
Correa P, Houghton J (2007) Carcinogenesis of Helicobacter pylori. Gastroenterology 133:659–672
Barros R, Camilo V, Pereira B et al (2010) Pathophysiology of intestinal metaplasia of the stomach: emphasis on CDX2 regulation. Biochem Soc Trans 38:358–363
Goldenring JR, Nomura S (2006) Differentiation of the gastric mucosa III Animal models of oxyntic atrophy and metaplasia. Am J Physiol Gastrointest Liver Physiol 291:G999–G1004
Fox JG, Rogers AB, Whary MT et al (2007) Accelerated Progression of Gastritis to Dysplasia in the Pyloric Antrum of TFF2−/− C57BL6 x Sv129 Helicobacter pylori-Infected Mice. Am J Pathol 171:1520–1528
Boivin GP, Washington K, Yang K et al (2003) Pathology of mouse models of intestinal cancer: consensus report and recommendations. Gastroenterology 124:762–777
Rogers AB, Taylor NS, Whary MT et al (2005) Helicobacter pylori but not high salt induces gastric intraepithelial neoplasia in B6129 mice. Cancer Res 65:10709–10715
Syder AJ, Oh JD, Guruge JL et al (2003) The impact of parietal cells on Helicobacter pylori tropism and host pathology: an analysis using gnotobiotic normal and transgenic mice. Proc Natl Acad Sci USA 100:3467–3472
Kang W, Rathinavelu S, Samuelson LC et al (2005) Interferon gamma induction of gastric mucous neck cell hypertrophy. Lab Invest 85:702–715
Fox JG, Feng Y, Theve EJ et al (2010) Gut microbes define liver cancer risk in mice exposed to chemical and viral transgenic hepatocarcinogens. Gut 59:88–97
Stolte M, Meining A (2001) The updated Sydney system: classification and grading of gastritis as the basis of diagnosis and treatment. Can J Gastroenterol 15:591–598
Fox JG, Rogers AB, Ihrig M et al (2003) Helicobacter pylori-associated gastric cancer in INS-GAS mice is gender specific. Cancer Res 63:942–950
Ward JM, Yoon M, Anver MR et al (2001) Hyalinosis and Ym1/Ym2 gene expression in the stomach and respiratory tract of 129 S4/SvJae and wild-type and CYP1A2-null B6, 129 mice. Am J Pathol 158:323–332
Haines DC, Chattopadhyay S, Ward JM (2001) Pathology of aging B6;129 mice. Toxicol Pathol 29:653–661
Cardiff RD, Ward JM, Barthold SW (2007) ‘One medicine-one pathology’: are veterinary and human pathology prepared? Lab Invest 88(1):18–26
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Rogers, A.B. (2012). Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models. In: Houghton, J. (eds) Helicobacter Species. Methods in Molecular Biology, vol 921. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-005-2_22
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DOI: https://doi.org/10.1007/978-1-62703-005-2_22
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-005-2
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