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Cardiovascular Development pp 147–154Cite as

Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 843))

Abstract

The formation of blood vessel networks is a fundamental event in vertebrate embryo development. Angiogenesis and vasculogenesis are the essential processes in vascular formation. Endothelial cells play a key role during angiogenesis and vasculogenesis, and cultured vascular endothelial cells provide an indispensable model for exploring the molecular mechanisms of angiogenesis and vasculogenesis. In this chapter, we described a protocol using PECAM-1-coated Dynabeads for the isolation of vascular endothelial cells from mouse heart and lung. This method will provide up to 107 endothelial cells with high purity (>85%). The isolated endothelial cells retain their in vivo characteristics, such as the expression of the cell surface markers PECAM-1 and ICAM-2.

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Acknowledgments

This work was supported by American Heart Association Scientist Development Grant and Texas A&M Health Science Center Startup Grant to Xu Peng.

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Authors and Affiliations

  1. Department of Systems Biology and Translational Medicine, College of Medicine, Texas A&M Healthy Science Center, Temple, TX, USA

    Yixin Jin, Yang Liu & Xu Peng

  2. Department of Molecular Medicine, School of Veterinary Medicine, Cornell University, Ithaca, NY, USA

    Marc Antonyak

Authors
  1. Yixin Jin
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  2. Yang Liu
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  3. Marc Antonyak
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  4. Xu Peng
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Corresponding author

Correspondence to Xu Peng .

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Editors and Affiliations

  1. College of Medicine, Dept. Systems Biology &, Texas A&M Health Science Center, SW. HK Dodgen Loop 702, Temple, 76504-7105, Texas, USA

    Xu Peng

  2. School of Veterinary Medicine, Department of Molecular Medicine, Cornell University, Ithaca, 14853, New York, USA

    Marc Antonyak

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© 2012 Springer Science+Business Media, LLC

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Jin, Y., Liu, Y., Antonyak, M., Peng, X. (2012). Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung. In: Peng, X., Antonyak, M. (eds) Cardiovascular Development. Methods in Molecular Biology, vol 843. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-523-7_14

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  • DOI: https://doi.org/10.1007/978-1-61779-523-7_14

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-522-0

  • Online ISBN: 978-1-61779-523-7

  • eBook Packages: Springer Protocols

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