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Meiosis pp 171–195Cite as

Cytological Analysis of Meiosis in Caenorhabditis elegans

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 558))

Abstract

The nematode Caenorhabditis elegans has emerged as an informative experimental system for analysis of meiosis, in large part because of the advantageous physical organization of meiotic nuclei as a gradient of stages within the germline. Here we provide tools for detailed observational studies of cells within the worm gonad, including techniques for light and electron microscopy.

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References

  1. Dernburg, A. F., Zalevsky, J., Colaiacovo, M. P., and Villeneuve, A. M. (2000) Transgene-mediated cosuppression in the C. elegans germ line. Genes Dev. 14, 1578–1583.

    PubMed  CAS  Google Scholar 

  2. Wilm, T., Demel, P., Koop, H. U., Schnabel, H., and Schnabel, R. (1999) Ballistic transformation of Caenorhabditis elegans. Gene 229, 31–35.

    Article  PubMed  CAS  Google Scholar 

  3. McDonald, K. (1999) High-pressure freezing for preservation of high resolution fine structure and antigenicity for immunolabeling. Methods Mol. Biol. 117, 77–97.

    Article  PubMed  CAS  Google Scholar 

  4. McDonald, K. L., Morphew, M., Verkade, P., and Muller-Reichert, T. (2007) Recent advances in high-pressure freezing: equipment- and specimen-loading methods. Methods Mol. Biol. 369, 143–173.

    Article  PubMed  CAS  Google Scholar 

  5. McDonald, K. (2007) Cryopreparation methods for electron microscopy of selected model systems. Methods Cell Biol. 79, 23–56.

    Article  PubMed  CAS  Google Scholar 

  6. Muller-Reichert, T., Hohenberg, H., O'Toole, E. T., and McDonald, K. (2003) Cryoimmobilization and three-dimensional visualization of C. elegans ultrastructure. J. Microsc. 212, 71–80.

    Article  PubMed  CAS  Google Scholar 

  7. Muller-Reichert, T., Srayko, M., Hyman, A., O'Toole, E. T., and McDonald, K. (2007) Correlative light and electron microscopy of early Caenorhabditis elegans embryos in mitosis. Methods Cell Biol. 79, 101–119.

    Article  PubMed  Google Scholar 

  8. McDonald, K. L. (1994) Electron microscopy and EM immunocytochemistry. Methods Cell Biol. 44, 411–444.

    Article  PubMed  CAS  Google Scholar 

  9. Walther, P., and Ziegler, A. (2002) Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water. J. Microsc. 208, 3–10.

    Article  PubMed  CAS  Google Scholar 

  10. MacQueen, A. J., Colaiacovo, M. P., McDonald, K., and Villeneuve, A. M. (2002) Synapsis-dependent and -independent mechanisms stabilize homolog pairing during meiotic prophase in C. elegans. Genes Dev. 16, 2428–2442.

    Article  PubMed  CAS  Google Scholar 

  11. MacQueen, A. J., and Villeneuve, A. M. (2001) Nuclear reorganization and homologous chromosome pairing during meiotic prophase require C. elegans chk-2. Genes Dev. 15, 1674–1687.

    Article  PubMed  CAS  Google Scholar 

  12. Lieb, J. D., de Solorzano, C. O., Rodriguez, E. G., Jones, A., Angelo, M., Lockett, S., and Meyer, B. J. (2000) The Caenorhabditis elegans dosage compensation machinery is recruited to X chromosome DNA attached to an autosome. Genetics 156, 1603–1621.

    PubMed  CAS  Google Scholar 

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Phillips, C.M., McDonald, K.L., Dernburg, A.F. (2009). Cytological Analysis of Meiosis in Caenorhabditis elegans . In: Keeney, S. (eds) Meiosis. Methods in Molecular Biology, vol 558. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-103-5_11

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  • DOI: https://doi.org/10.1007/978-1-60761-103-5_11

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-102-8

  • Online ISBN: 978-1-60761-103-5

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