Abstract
In malaria vaccine research, one of the major obstacles has been the difficulty of expressing recombinant malarial proteins and it is mainly due to the lack of an efficient methodology for the synthesis of sufficient quantity of quality proteins. We demonstrate that the wheat germ cell-free protein synthesis system can be applied for the successful production of leading malaria vaccine candidate antigens and, thus, prove that it may be a key tool for malaria vaccine research.
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Acknowledgments
This work was supported in part by Grants-in-Aid for Scientific Research 18390129 and 19406009; Scientific Research on Priority Areas 21022034 from the Ministry of Education, Culture, Sports, Science, and Technology, Japan; and, in part, by a Grant-in-Aid of the Ministry of Health, Labour, and Welfare (H21-Chikyukibo-ippan-005), Japan.
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Tsuboi, T., Takeo, S., Sawasaki, T., Torii, M., Endo, Y. (2010). An Efficient Approach to the Production of Vaccines Against the Malaria Parasite. In: Endo, Y., Takai, K., Ueda, T. (eds) Cell-Free Protein Production. Methods in Molecular Biology, vol 607. Humana Press. https://doi.org/10.1007/978-1-60327-331-2_8
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DOI: https://doi.org/10.1007/978-1-60327-331-2_8
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