Abstract
Drosophila pupal (P) wing development entails a series of dynamic developmental events, such as epithelial and glial morphogenesis, that are of outstanding interest to cell biologists. Here, we first describe how to prepare P and prepupal (PP) wings for immunofluorescence microscopy. This protocol has been optimized to visualize wing epithelial architecture, such as polarized cortical domains of planar cell polarity proteins. We then provide a protocol to prepare pupae for whole mount live imaging of P wings. This procedure has allowed us to live-image glial cell migration and proliferation along wing sensory nerves.
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© 2008 Humana Press Inc., Totowa, NJ
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Classen, AK., Aigouy, B., Giangrande, A., Eaton, S. (2008). Imaging Drosophila Pupal Wing Morphogenesis. In: Dahmann, C. (eds) Drosophila. Methods in Molecular Biology, vol 420. Humana Press. https://doi.org/10.1007/978-1-59745-583-1_16
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DOI: https://doi.org/10.1007/978-1-59745-583-1_16
Publisher Name: Humana Press
Print ISBN: 978-1-58829-817-1
Online ISBN: 978-1-59745-583-1
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