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T-Cell Motility pp 99–113Cite as

Profiling Activity of Cellular Kinases in Migrating T-Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1930))

Abstract

T-Lymphocyte kinases are important checkpoints that control T-cell motility by regulating a diverse range of signal transduction pathways. The distinct configuration of kinase events in T-cell could be used to fingerprint the status of T-cells. However, only small fraction human kinases have been characterized so far and little is known about the dynamics of the kinome in motile T-cells. Although several direct and indirect strategies exist to characterize cellular kinase activities, such as RNA interference, antibody arrays, enzyme kinetics, and mass spectrometry, this chapter focuses on an alternative multiplex phosphopeptide array-based methodology, which allows the kinome-wide identification of hyper-activated kinases involved in the regulation of T-cell migration.

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Acknowledgements

The authors acknowledge funding support from the Lee Kong Chian School of Medicine, Nanyang Technological University Singapore Start-Up Grant to N.K.V. and the Ministry of Education (MOE) Singapore under its Singapore MOE Academic Research Fund (AcRF) Tier 1 (2014-T1-001-141) and MOE-AcRF Tier 2 (MOE2017-T2-2-004) grants and Hercules Grant, U Antwerp to W.V.B.

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Authors and Affiliations

  1. Laboratory of Protein Chemistry, Proteomics and Epigenetic Signalling (PPES), Department of Biomedical Sciences, University of Antwerp (UA), Antwerpen, Belgium

    Chandra Sekhar Chirumamilla, Claudina Perez-Novo, Padma Ramireddy & Wim Vanden Berghe

  2. Lymphocyte Signalling Research Laboratory, Lee Kong Chain School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore

    Mobashar Hussain Urf Turabe Fazil

  3. PamGene International B.V., 5200 BJ ’s-Hertogenbosch, The Netherlands

    Savithri Rangarajan & Rik de Wijn

  4. Lee Kong Chain School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore

    Navin Kumar Verma

Authors
  1. Chandra Sekhar Chirumamilla
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  2. Mobashar Hussain Urf Turabe Fazil
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  3. Claudina Perez-Novo
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  4. Savithri Rangarajan
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  5. Rik de Wijn
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  6. Padma Ramireddy
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  7. Navin Kumar Verma
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  8. Wim Vanden Berghe
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Corresponding author

Correspondence to Mobashar Hussain Urf Turabe Fazil .

Editor information

Editors and Affiliations

  1. Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore

    Navin Kumar Verma

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Chirumamilla, C.S. et al. (2019). Profiling Activity of Cellular Kinases in Migrating T-Cells. In: Verma, N. (eds) T-Cell Motility. Methods in Molecular Biology, vol 1930. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9036-8_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9036-8_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9035-1

  • Online ISBN: 978-1-4939-9036-8

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