Abstract
Multiparameter flow cytometry of human lungs allows for characterization, isolation, and examination of human pulmonary immune cell composition, phenotype, and function. Here we describe an approach to process lung tissues and then utilize a base antibody panel to define all of the major immune cell types in a single staining condition. This base antibody panel can also be used to identify major immune cell types in human blood and bronchoalveolar lavage (BAL) fluid.
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Acknowledgment
The work is supported by NIH grants K08 HL121185, K08 HL105537, Mandel Fellowship Award, and Proof of Concept Award from the Pulmonary Hypertension Association.
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Yu, YR.A., Tighe, R.M. (2018). Isolation and Characterization of Human Lung Myeloid Cells. In: Alper, S., Janssen, W. (eds) Lung Innate Immunity and Inflammation. Methods in Molecular Biology, vol 1809. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8570-8_9
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DOI: https://doi.org/10.1007/978-1-4939-8570-8_9
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