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Esophageal Adenocarcinoma pp 187–194Cite as

Liquid Biopsy for Investigation of Cancer DNA in Esophageal Adenocarcinoma: Cell-Free Plasma DNA and Exosome-Associated DNA

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1756))

Abstract

Liquid biopsy of cancers is an area of increasing interest in medical practice for the surveillance, management, and potential detection of malignant cells, using minimally invasive collection of body fluids. A liquid biopsy is particularly useful for metastatic cancers, which may be difficult to be sampled by core biopsy, due to difficulty of access or an occult location. Access to DNA shed from esophageal adenocarcinoma can enable the detection of mutations confirming the presence of malignant cells or the evolution of clonal lines with altered treatment response profiles. In this chapter, we detail a method for the isolation of cell-free DNA from blood plasma and DNA associated with exosomes in blood from patients with esophageal adenocarcinoma.

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Author information

Authors and Affiliations

  1. Genomics Research Centre, Institute of Health and Biomedical Innovation, School of Biomedical Science, Queensland University of Technology, Kelvin Grove, QLD, Australia

    Robert A. Smith

  2. Cancer Molecular Pathology of School of Medicine, Griffith University, Gold Coast, Australia

    Alfred K. Lam

Authors
  1. Robert A. Smith
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  2. Alfred K. Lam
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Corresponding author

Correspondence to Robert A. Smith .

Editor information

Editors and Affiliations

  1. Cancer Molecular Pathology of School of Medicine, Griffith University, Gold Coast, Australia

    Alfred K. Lam

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Smith, R.A., Lam, A.K. (2018). Liquid Biopsy for Investigation of Cancer DNA in Esophageal Adenocarcinoma: Cell-Free Plasma DNA and Exosome-Associated DNA. In: Lam, A. (eds) Esophageal Adenocarcinoma. Methods in Molecular Biology, vol 1756. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7734-5_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7734-5_17

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7733-8

  • Online ISBN: 978-1-4939-7734-5

  • eBook Packages: Springer Protocols

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