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Characterization of Circular RNA Concatemers

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Circular RNAs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1724))

Abstract

Circular RNAs (circRNAs) constitute a novel subset in the fascinating world of noncoding RNA, and they are found in practically all eukaryotes. Most of them exhibit low expression levels but some are extremely abundant. Typically, circRNAs are studied by RT-PCR-based assays, but for certain types of analyses this technique is not suitable. Circular RNA with repetitive exons (circular concatemers) has been observed by us and others when transiently expressing circRNAs in cells, however techniques and assays to study these species have not been established. Here, this chapter outlines three biochemical assays (RNase R-, RNase H-, and alkaline-treatment) that combined with northern blotting are useful to study circRNAs in general and circular RNA concatemers in particular.

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Acknowledgments

This work was supported by the Novo Nordisk Foundation (NNF16OC0019874).

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics (MBG) and Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark

    Thomas B. Hansen

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  1. Thomas B. Hansen
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Correspondence to Thomas B. Hansen .

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Editors and Affiliations

  1. Department of Internal Medicine III Klaus Tschira Institute for Integrative Computational Cardiology, University Hospital Heidelberg, Heidelberg, Germany

    Christoph Dieterich

  2. Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany

    Argyris Papantonis

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Hansen, T.B. (2018). Characterization of Circular RNA Concatemers. In: Dieterich, C., Papantonis, A. (eds) Circular RNAs. Methods in Molecular Biology, vol 1724. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7562-4_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7562-4_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7561-7

  • Online ISBN: 978-1-4939-7562-4

  • eBook Packages: Springer Protocols

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