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Analysis of Yeast Mitochondria by Electron Microscopy

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Book cover Mitochondria

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1567))

Abstract

Budding yeast Saccharomyces cerevisiae represents a widely used model organism for the study of mitochondrial biogenesis and architecture. Electron microscopy is an essential tool in the analysis of cellular ultrastructure and the precise localization of proteins to organellar subcompartments. We provide here detailed protocols for the analysis of yeast mitochondria by transmission electron microscopy: (1) chemical fixation and Epon embedding of yeast cells and isolated mitochondria, and (2) cryosectioning and immunolabeling of yeast cells and isolated mitochondria according to the Tokuyasu method.

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Acknowledgments

We thank Fulvio Reggiori and Muriel Mari (University Medical Centre Utrecht, The Netherlands) for advice and excellent training in cryo-sectioning, Rita Grotjahn for technical assistance, and Anja Heublein for photography. This work was supported by the Elitenetzwerk Bayern and a Max-Planck-Fellowship to A.-K.U. and grants of the Deutsche Forschungsgemeinschaft to B.W.

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Authors and Affiliations

  1. Institut für Zellbiologie, Universität Bayreuth, 95440, Bayreuth, Germany

    Ann-Katrin Unger, Stefan Geimer & Benedikt Westermann

  2. Max Planck Institut für Biochemie, Am Klopferspitz 18, 82152, Martinsried, Germany

    Max Harner & Walter Neupert

Authors
  1. Ann-Katrin Unger
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  2. Stefan Geimer
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  3. Max Harner
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  4. Walter Neupert
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  5. Benedikt Westermann
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Corresponding author

Correspondence to Benedikt Westermann .

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Editors and Affiliations

  1. Biomedical Center-Physiological Chemistry, LMU Munich, Martinsried, Germany

    Dejana Mokranjac

  2. Gene Center, LMU Munich and Institute for Diabetes and Obesity, Helmholtz Zentrum München, Munich, Germany

    Fabiana Perocchi

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Unger, AK., Geimer, S., Harner, M., Neupert, W., Westermann, B. (2017). Analysis of Yeast Mitochondria by Electron Microscopy. In: Mokranjac, D., Perocchi, F. (eds) Mitochondria. Methods in Molecular Biology, vol 1567. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6824-4_18

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  • DOI: https://doi.org/10.1007/978-1-4939-6824-4_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6822-0

  • Online ISBN: 978-1-4939-6824-4

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