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Zebrafish pp 207–223Cite as

Live Imaging of Host–Pathogen Interactions in Zebrafish Larvae

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1451))

Abstract

Zebrafish larvae are a powerful platform for studying the innate immune response to infection. The small size and optical transparency of larval zebrafish allow for multiple subject, multidimensional, and longitudinal imaging experiments. This chapter describes protocols for infecting zebrafish larvae with their natural pathogen Mycobacterium marinum, rapid short-term imaging, long-term extended imaging, and drug treatment assays. These protocols can be easily adapted to image and manipulate host interactions with other pathogens.

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Acknowledgments

We thank Dana Sisk for expert management of our zebrafish facility and Rebecca Beerman, Mark Cronan, and Kevin Takaki for technical advice. This work was supported by funding from a National Science Foundation Graduate Research Fellowship (M.A.M.), an Australian National Health and Medical Research Council CJ Martin Early Career Fellowship (S.H.O.), and a Mallinckrodt Scholar Award, a Searle Scholar Award, a Vallee Foundation Young Investigator Award, an NIH Director’s New Innovator Award (1DP2-OD008614), and the Duke University Center for AIDS Research, a National Institutes of Health (NIH)-funded program (5P30 AI064518) (D.M.T.). The authors declare no conflict of interest.

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    Authors and Affiliations

    1. Department of Molecular Genetics and Microbiology, Center for Host-Microbial Interactions, Duke University Medical Center, 219 Jones Building, Box 3020 DUMC, Durham, NC, 27710, USA

      Molly A. Matty, Stefan H. Oehlers & David M. Tobin

    2. University Program in Genetics and Genomics, Duke University, Durham, NC, 27710, USA

      Molly A. Matty

    3. Department of Immunology, Duke University Medical Center, Durham, NC, 27710, USA

      David M. Tobin

    Authors
    1. Molly A. Matty
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    2. Stefan H. Oehlers
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    3. David M. Tobin
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    Corresponding author

    Correspondence to David M. Tobin .

    Editor information

    Editors and Affiliations

    1. Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Japan

      Koichi Kawakami

    2. Institute of Genetics and Molecular Medicine, MRC Human Genetics Unit & The University of Edinburgh Cancer Research UK Centre, Edinburgh, United Kingdom

      E. Elizabeth Patton

    3. Champalimaud Centre for the Unknown, Champalimaud Neuroscience Programme Champalimaud Centre for the Unknown, Lisbon, Portugal

      Michael Orger

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    © 2016 Springer Science+Business Media New York

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    Matty, M.A., Oehlers, S.H., Tobin, D.M. (2016). Live Imaging of Host–Pathogen Interactions in Zebrafish Larvae. In: Kawakami, K., Patton, E., Orger, M. (eds) Zebrafish. Methods in Molecular Biology, vol 1451. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3771-4_14

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    • DOI: https://doi.org/10.1007/978-1-4939-3771-4_14

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    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-3769-1

    • Online ISBN: 978-1-4939-3771-4

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