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Import of Proteins into Isolated Yeast Mitochondria

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Book cover Membrane Trafficking

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1270))

Abstract

Mitochondria are essential organelles of eukaryotic cells. The vast majority of mitochondrial proteins is encoded within the nuclear genome and translocated into various mitochondrial compartments after translation in the cytosol as preproteins. Even in rather primitive eukaryotes like yeasts, there are 700–1,000 different proteins that need to be recognized in the cytosol, directed to the protein translocases in the two mitochondrial membranes and sorted to their appropriate mitochondrial subcompartment. In vitro reconstituted import systems have proved to be important tools to study these processes in detail. Using isolated mitochondria and radioactively labeled precursor proteins, it was possible to identify several import machineries and pathways consisting of a large number of components during the last few decades.

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Acknowledgments

This work was supported by grants of the Deutsche Forschungsgemeinschaft and the Landesschwerpunkt BioComp of Rheinland-Pfalz.

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Authors and Affiliations

  1. Cell Biology, University of Kaiserslautern, Erwin-Schrodinger-Strasse 13, 67663, Kaiserslautern, Germany

    Valentina Peleh, Ajay Ramesh & Johannes M. Herrmann

Authors
  1. Valentina Peleh
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  2. Ajay Ramesh
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  3. Johannes M. Herrmann
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Corresponding author

Correspondence to Johannes M. Herrmann .

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Editors and Affiliations

  1. Department of Biochemistry, National University of Singapore, Singapore, Singapore

    Bor Luen Tang

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Peleh, V., Ramesh, A., Herrmann, J.M. (2015). Import of Proteins into Isolated Yeast Mitochondria. In: Tang, B. (eds) Membrane Trafficking. Methods in Molecular Biology, vol 1270. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2309-0_3

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  • DOI: https://doi.org/10.1007/978-1-4939-2309-0_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2308-3

  • Online ISBN: 978-1-4939-2309-0

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