Regular ArticlePlasmid R1 Is Present as Clusters in the Cells of Escherichia coli
References (20)
- et al.
Chromosome and low copy plasmid segregation in E. coli: Visual evidence for distinct mechanisms
Cell
(1997) - et al.
Selection and timing of replication of plasmids R1drd-19 and F′lac in Escherichia coli
Plasmid
(1978) - et al.
Subcellular distribution of actively partitioning F plasmid during the cell division cycle in E. coli
Cell
(1997) - et al.
Kinetic aspects of control of plasmid replication by antisense RNA
TIBS
(1994) - et al.
Partitioning of plasmid R1 in Escherichia coli. I. Kinetics of loss of plasmid derivatives deleted of the par region
Plasmid
(1980) - et al.
Partitioning of plasmid R1 in Escherichia coli. II. Incompatibility properties of the partitioning system
Plasmid
(1980) - et al.
Plasmid R1 in Salmonella typhimurium: Molecular instability and gene dosage effects
Plasmid
(1979) - et al.
R plasmid gene dosage effects in Escherichia coli K12: Copy mutants of the R plasmid R1drd-19
Plasmid
(1977) - et al.
Cell division in Escherichia coli minB mutants
Mol. Microbiol.
(1992)
Cited by (42)
Organization of ribosomes and nucleoids in escherichia coli cells during growth and in quiescence
2014, Journal of Biological ChemistryCitation Excerpt :The notion of structureless, homogeneous bacterial cytoplasm through which macromolecules diffuse freely to interact by random collisions has been replaced by a system of macromolecular machines designed for specific functions assembled at specific locations at appropriate times such that growth, replication, and cell division processes function in coordination to maintain remarkably error-free cycles of growth and reproduction for generations (1–3). This was also suspected earlier with the discovery of plasmids clustered at characteristic intracellular positions (4, 5) and the sequential movement of the bacterial chromosomes during replication (6–9). Now it has been revealed that the bacterial interior possesses a highly ordered subcellular architecture comprising dynamic networks of cytoskeletal fibers (10–12), multiprotein complexes constituting replication, transcription, and translation machineries assembled at characteristic locations (13–19), and oscillatory relocalization of protein complexes in defined trajectories resulting in concentration gradients (20, 21).
Eclipse period of R1 plasmids during downshift from elevated copy number: Nonrandom selection of copies for replication
2012, PlasmidCitation Excerpt :This, however, is unlikely as this rare transition was observed only when the ter site in the oriR1 was rendered inactive either by changes in the ter-sequence or in a Tus−-host; neither of which applied above. Plasmid R1, like many other plasmids, forms clusters containing more than one plasmid copies (Eliasson et al., 1992; Weitao et al., 2000; Pogliano, 2002). The average number of clusters for the wild type and a cop mutant of R1 with about fourfold higher copy number was found to be the same; the clusters for the copy number mutants appearing brighter due to higher plasmid DNA content (Weitao et al., 2000).
Evaluating quantitative methods for measuring plasmid copy numbers in single cells
2012, PlasmidCitation Excerpt :In lieu of that, it may be sufficient to compare the average copy number to bulk methods, keeping in mind that outliers contribute much more to standard deviations than they do to averages. Plasmid FISH has been used by several groups, including Kurt Nordström (Weitao et al., 2000), for measuring the localization of plasmids but has not been published as a means to count plasmids. Plasmid FISH suffers from all the issues mentioned above plus one more: the DNA strands must be separated to allow the probe to bind, usually by denaturation at high temperature.
Plasmid Segregation: Is a Total Understanding within Reach?
2008, Current BiologyCitation Excerpt :And how does the interaction with ParR-parC stabilize ParM filaments? How can one spindle consist of multiple ParM filaments, as Campbell and Mullins [4] demonstrated, and are the observed ‘plasmids’ actually clusters of multiple plasmids [12]? Moving up in complexity, it is not obvious that the proposed search-and-capture mechanism can account for all the observed interactions between plasmids.
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