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Plasmodium bergheiANKA: Purification of Large Numbers of Infectious Gametocytes

https://doi.org/10.1006/expr.1998.4203Get rights and content

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    To purify gametocytes, infected mice (∼15 % parasitemia) were treated with 20 mg/L sulfadiazine (Sigma) in drinking water for 48 h to kill off asexual blood stages. Gametocytes were fractionated on a 48 % (v/v) Nycodenz gradient at 4 °C to avoid activation of gametocytes [34]. Early asexual stage parasites (ring-trophozoite) were obtained after removal of gametocytes and schizonts using 48 % (v/v) and 55 % (v/v) Nycodenz gradients.

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