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Increased Transcript Level of RBM3, a Member of the Glycine-Rich RNA-Binding Protein Family, in Human Cells in Response to Cold Stress

https://doi.org/10.1006/bbrc.1997.7059Get rights and content

Abstract

Although the cold-shock responses of microorganisms have been extensively investigated, those of mammalian cells are just beginning to be understood. Recently, CIRP, a member of the glycine-rich RNA-binding protein (GRP) family, has been identified as the first cold-shock protein in mammalian cells. Here, we report that RBM3, another member of the GRP family, is induced in human cells in response to cold stress (32°C).RBM3transcripts were constitutively expressed in all cell lines examined including K562, HepG2, NC65, HeLa, and T24 cells. In all of them, the transcript levels ofRBM3were increased at 24 h after the 37 to 32°C temperature down-shift. In NC65 cells, the kinetics ofRBM3induction was different from that ofCIRP.Protein synthesis inhibitors cycloheximide and puromycin inducedRBM3transcripts, but cadmium chloride, H2O2, ethanol, and osmotic shock had no effect. Combined with the different tissue distribution of expression, these results suggest that RBM3 and CIRP play distinct roles in cold responses of human cells.

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    Abbreviations: RNP, ribonucleoproteinCS-RBD, consensus sequence-RNA-binding domain; GRP, glycine-rich RNA-binding protein;

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