Immunology
Viperin interacts with PEX19 to mediate peroxisomal augmentation of the innate antiviral responseThis work highlights the multifunctional role of the ISG viperin and its interaction with the peroxisomal protein Pex19 to modulate peroxisomal-dependent innate signaling that ultimately restricts viral infection.
Human IFITM3 restricts chikungunya virus and Mayaro virus infection and is susceptible to virus-mediated counteractionEndogenous and heterologpusly expressed human IFITM3 restricts early and late replication steps of chikungunya and Mayaro virus infection and is downregulated at the protein level in infected cells.
Single-cell RNA sequencing of mouse islets exposed to proinflammatory cytokinesSingle-cell RNA-seq was used to show that islet endocrine cells respond to acute cytokine exposure with an increase in the expression of protective genes and the absence of apoptotic gene expression.
NF-κB inhibition in keratinocytes causes RIPK1-mediated necroptosis and skin inflammationTNFR1 was found to cause skin inflammation in mice with epidermal keratinocyte-specific ablation of IKK2 or of both the NF-kB subunits RelA and cRel by triggering RIPK1-dependent, RIPK3-MLKL–mediated necroptosis of keratinocytes.
Proteomics characterisation of the L929 cell supernatant and its role in BMDM differentiationL929 cell supernatant is commonly used to differentiate murine macrophages from bone marrow. The supernatant and its effect on macrophage phenotype was characterised by proteomics and the authors identified novel immunoregulatory proteins.
DGCR8 deficiency impairs macrophage growth and unleashes the interferon response to mycobacteriaLack of miRNA biosynthesis in DGCR8-deficient macrophages did not block macrophage differentiation but caused overshooting type I IFN responses to mycobacterial glycolipids.
Dendritic cell entry to lymphatic capillaries is orchestrated by CD44 and the hyaluronan glycocalyxCD44 anchors the hyaluronan glycocalyx on migrating dendritic cells to permit docking to the endothelial receptor LYVE-1, thus orchestrating lymphatic trafficking through modulating glycocalyx density.
Renal CD169++ resident macrophages are crucial for protection against acute systemic candidiasisA renal CD169++ resident macrophage subpopulation controls Candida albicans growth, severe kidney inflammation, and immunopathology during acute systemic candidiasis.
Short-term IL-15 priming leaves a long-lasting signalling imprint in mouse NK cells independently of a metabolic switchNK cell reactivity is dynamically regulated by IL-15, and NK cells do not need more than a few minutes of exposure to remember the cytokine for several hours.
Profiling of immune dysfunction in COVID-19 patients allows early prediction of disease progressionDeep immune profiling of COVID-19 patients with different disease severity reveals populations associated with severe disease and can be used to predict disease severity early on.