Immunology
Proteomics characterisation of the L929 cell supernatant and its role in BMDM differentiationL929 cell supernatant is commonly used to differentiate murine macrophages from bone marrow. The supernatant and its effect on macrophage phenotype was characterised by proteomics and the authors identified novel immunoregulatory proteins.
DGCR8 deficiency impairs macrophage growth and unleashes the interferon response to mycobacteriaLack of miRNA biosynthesis in DGCR8-deficient macrophages did not block macrophage differentiation but caused overshooting type I IFN responses to mycobacterial glycolipids.
Dendritic cell entry to lymphatic capillaries is orchestrated by CD44 and the hyaluronan glycocalyxCD44 anchors the hyaluronan glycocalyx on migrating dendritic cells to permit docking to the endothelial receptor LYVE-1, thus orchestrating lymphatic trafficking through modulating glycocalyx density.
Renal CD169++ resident macrophages are crucial for protection against acute systemic candidiasisA renal CD169++ resident macrophage subpopulation controls Candida albicans growth, severe kidney inflammation, and immunopathology during acute systemic candidiasis.
Short-term IL-15 priming leaves a long-lasting signalling imprint in mouse NK cells independently of a metabolic switchNK cell reactivity is dynamically regulated by IL-15, and NK cells do not need more than a few minutes of exposure to remember the cytokine for several hours.
Profiling of immune dysfunction in COVID-19 patients allows early prediction of disease progressionDeep immune profiling of COVID-19 patients with different disease severity reveals populations associated with severe disease and can be used to predict disease severity early on.
Sustained Helicobacter pylori infection accelerates gastric dysplasia in a mouse modelHp infection was found to alter metaplasia and accelerate dysplasia in mice. Thus, Hp can promote gastric preneoplastic progression, in addition to its known role in initiating chronic inflammation.
Meningeal lymphoid structures are activated under acute and chronic spinal cord pathologiesWe found that acute insult to the central nervous system induces the formation of lymphocyte aggregates reminiscent of tertiary lymphoid structures within the spinal cord meninges. Unlike draining CNS-cervical lymph nodes, meningeal lymphocytes are locally activated during neuro-inflammtion and neurodegeneration.
Single-cell RNA sequencing of human breast tumour-infiltrating immune cells reveals a γδ T-cell subtype associated with good clinical outcomeSingle-cell sequencing of γδ-T cells from human blood and tumours revealed novel markers of subtypes with distinct effector functions and a subtype that is associated with favourable clinical outcome.
G-CSF shifts erythropoiesis from bone marrow into spleen in the setting of systemic inflammationGranulocyte colony-stimulating factor suppresses erythropoiesis of bone marrow and promotes splenic erythropoiesis during lipopolysaccharide-induced systemic inflammation in mice.