Table 1.

Kinetic parameters of monobodies against the SARS-CoV-2 wild-type receptor-binding domain determined by BLI.

NamesBC loopFG loopKD (nM)kon (1/Ms) × 105koff (1/s) × 10−4
C4PSSRYEHYQFWTGDVPWYWLVN0.962.42.3
C4-AM1PRGGPEHYQFWTGDVPWYWSRN0.113.30.38
C4-AM2PRGGPADYQWWTGKVPWYWSAN<0.012.3<0.01
C6bGGDYVGYYTYNGPWIYGYEEI0.511.20.63
C6b-AM1GGAGAHLYTYNGPWIYGYEEI1.71.22.0
C6b-GSGSGSGSTYNGPWIYGYEEI4.31.35.6
C6b-PAVTPAVTTYNGPWIYGYEEI7.21.611
C6b-AM2aWIMQLDSGYWDRTYNGPWIYGYEEI0.360.950.34
C12bEIYYEIGDRLWGYYTQWD0.671.71.1
C12b-AM1GLGSSFGDRLWGYYTQWD1.01.91.9
C12b-AM2aMHWYDQGDTSRLWGYYTQWD<0.011.6<0.01
  • a The C6b-AM2 and C12b-AM2 monobodies had two amino acids (VR) deletion after the BC loop.