Table 3.

QuPath and manual counting of positive Bruton tyrosine kinase protein staining, positive Bruton tyrosine kinase protein negative staining, total staining count, the percentage of positive staining, and the relative error (RE) between the two methods.

Core numberGradePositive countNegative countTotal countPercentage of positive
QuPathManualREQuPathManualREQuPathManualREQuPathManualRE
1184788790−0.0031,7401,923−0.0952,5282,713−0.06831.229.10.070
10141,1181,191−0.0612,1352,664−0.1993,2533,855−0.15634.430.90.112
1352865980−0.117104850.2249691,065−0.09089.392.0−0.030
1402264270−0.0221,6211,677−0.0331,8851,947−0.03214.013.90.010
163238271−0.1226526320.032890903−0.01426.730.0−0.109
1533642767−0.1632,5172,957−0.1493,1593,724−0.15220.320.6−0.013
144446478−0.0672,5282,703−0.0652,9743,181−0.06515.015.0−0.002
4744343700.173814897−0.0931,2481,267−0.01534.829.20.191
564801830−0.0351,5831,695−0.0662,3842,525−0.05633.632.90.022
6949439230.022248255−0.0271,1911,1780.01179.278.40.011
724694713−0.0271,7591,6760.0502,4532,3890.02728.329.8−0.052
8145495080.0819401,037−0.0941,4891,545−0.03636.932.90.121
8941,3051,339−0.0251,6332,115−0.2282,9383,454−0.14944.438.80.146
  • Red values represent the real difference between the methods. QP, QuPath; Man, manual; RE, relative error.