Protocol for H&E staining.
Cycles | Solution | Time |
---|---|---|
1× | Mayer’s Hematoxylin solution | 3 min |
3× | ddH2O | 10 s |
1× | Scott water | 30 s |
1× | ddH2O | 10 s |
1× | 70% (v/v) ethanol/ddH2O | 10 s |
1× | Eosin solution | 1 min |
2× | 80% (v/v) ethanol/ddH2O | 10 s |
2× | 95% (v/v) ethanol/ddH2O | 10 s |
2× | Ethanol | 10 s |
2× | Xylene | 10 min |
Slides were finally mounted with Eukitt and air-dried |