Protocol for H&E staining.

CyclesSolutionTime
Mayer’s Hematoxylin solution3 min
ddH2O10 s
Scott water30 s
ddH2O10 s
70% (v/v) ethanol/ddH2O10 s
Eosin solution1 min
80% (v/v) ethanol/ddH2O10 s
95% (v/v) ethanol/ddH2O10 s
Ethanol10 s
Xylene10 min
Slides were finally mounted with Eukitt and air-dried