Protocol for H&E staining.
| Cycles | Solution | Time |
|---|---|---|
| 1× | Mayer’s Hematoxylin solution | 3 min |
| 3× | ddH2O | 10 s |
| 1× | Scott water | 30 s |
| 1× | ddH2O | 10 s |
| 1× | 70% (v/v) ethanol/ddH2O | 10 s |
| 1× | Eosin solution | 1 min |
| 2× | 80% (v/v) ethanol/ddH2O | 10 s |
| 2× | 95% (v/v) ethanol/ddH2O | 10 s |
| 2× | Ethanol | 10 s |
| 2× | Xylene | 10 min |
| Slides were finally mounted with Eukitt and air-dried | ||