Table 1.

Inflexion temperature of SYT1C2A and SYT1C2B in the presence 20 μM EGTA and different lipid polar heads.

Lipid polar headsSYT1C2AC2APolyBSYT1C2B
Control57.4 ± 0.561.4 ± 0.151 ± 1
IP259.4 ± 0.261.5 ± 0.1
IP362.60 ± 0.0561.4 ± 0.151 ± 1
IP(1,3,4)64.2 ± 0.161.4 ± 0.3
IP(1,4,5,6)62.6 ± 0.261.5 ± 0.3
IP(3,4,5,6)65.2 ± 0.461.4 ± 0.3
Ins56.8 ± 0.2
  • IP2, inositol 1,4-biphosphate; IP3, inositol 1,4,5-trisphosphate; IP(1,3,4), inositol 1,3,4-trisphosphate; IP(1,4,5,6), inositol 1,4,5,6-tetraphosphate; IP(3,4,5,6), inositol 3,4,5,6-tetraphosphate; Ins, inositol. The unbound protein is taken as a control.