List of IFITM3 variants generated by gene editing of HeLa cells
| IFITM3 clone | Type of gene edit | Gene edit (nt position refers to NM_021034.3) | Genomic DNA sequence | Guide RNA target (PAM) |
|---|---|---|---|---|
| KO | +1 frameshift | Insertion of A between nts 84 and 85 | CCTGTCA[+A]ACAGTGGCCAG | GGGGGCTGGCCACTGTTGAC(AGG) |
| Δexon1 | Deletion | Deletion of 381 nts post nt 36 | CGACCGCCGCTGGTCTT [deletion of 381 nt] | 1:CGACCGCCGCTGGTCTTCGC(TGG) |
| TCCCGTGTGTGCCCACG | ||||
| 2:CGTGGGCACACACGGGACAG(AGG) | ||||
| SNP rs12252-C | T to C transition | T89C | CCTGTCAACAGCGGCCAGCCCCC | guide:GGGGGCTGGCCACTGTTGAC(AGG) |
| +HDR-Donor: CTGGACACCATGAATCACACTGTCCAAACCTTCTTCTCTCCTGT | ||||
| CAACAGCGGCCAGCCCCCCAACTATGAGATGCTCAAGGAGGAGCAC | ||||
| Δ1st ATG | Deletion | Deletion of 31 nts post nt 36 | CGACCGCCGCTGGTCTT [deletion of 31 nt] | 1:CGACCGCCGCTGGTCTTCGC(TGG) |
| 2:TGACAGGAGAGAAGAAGGTT(TGG) | ||||
| CTTCTTCTCTCCTGTCAA |
nt, nucleotide.