Table 3.

List of antibodies used for immunogold EM.

Antibodies used for immunogold labelingRRID (−), not found or no exact matchCatalog or clone no.CompanyMono or polyAnimals for antibody productionDilution used
Anti-tubuinNot registeredaProduced by Dr. Fujii, Shinshu UniversityPolyclonalRabbit1/20
Anti-α-tubulin(−)RB9281-P0Thermo Fisher Scientific K.K.PolyclonalRabbit1/20
Anti-β-tubulinAB_609915T-5201Sigma-AldrichMonoclonalMouse1/20
Anti-βIII-tubulin(−)MMS-435PCovanceMonoclonalMouse1/20
Anti-β-actinbAB_626632sc-47778Santa Cruz Biotechnology Inc.MonoclonalMouse1/20
Anti-α-internexinAB_91800AB5354Chemicon InternationalPolyclonalRabbit1/500c
Anti-spectrin (nonerhtyroid)dAB_11214057MAB1622Chemicon InternationalMonoclonalMouse1/20
Anti-CaMKIIα(−)6G9Chemicon InternationalMonoclonalMouse1/20
Anti-CaMKIIβ(−)3232SAGibco BRLMonoclonalMouse1/20
Anti-PSD-95(−)MA1-045ABRMonoclonalMouse1/20
Anti-Homer 1AB_1950505GTX103278GeneTex, Inc.PolyclonalRabbit1/20
Anti-shank1AB_2270283N22/21UC Davls/NIH NeuroMab facilityMonoclonalMouse1/20
Anti-GKAP (Pan-SAPAP)ABJ0671947N127/31UC Davls/NIH NeuroMab facilityMonoclonalMouse1/20
Anti-ATP5A1, C-termAB_10618791GTX101741GeneTex, Inc.PolyclonalRabbit1/20
Anti-ANTAB_671086sc-9299Santa Cruz Biotechnology Inc.PolyclonalGoat1/20
Anti-Mouse IgG (H+L)-gold label(−)EMGMHL5, EMGMHL10BBI solutionsPolyclonalGoat1/50
Anti-Rabbit IgG (H+L)-gold label(−)EMGAR5, EMGAR10BBI solutionsPolyclonalGoat1/50
Anti-Goat IgG (H+L)-gold label(−)EMRAG10BBI solutionsPolyclonalRabbit1/50
  • a Anti-tubulin antibody was produced in rabbit using pig tubulin as antigen, and affinity-purified (Liu et al, 2013).

  • b Anti-β-actin (sc-47778) was used because anti-pan-actin antibody (pan Ab-5; Thermo Fisher Scientific, UK, RRID, AB_10983629) did not label well the 1% OG-11B and PSD.

  • c This dilution is due to the stock solution which is 1/100 diluent of the original solution.

  • d Anti-fodrin.